Species & Dataset

  • Populus tremuloide

  • Common name: Trembling poplar, Golden aspen

  • Family: Salicaceae

Foliar Ozone Injury

Experiment

  • Cultivar: Populus tremuloide clone 216

  • Tissue: Shoot leaves

  • Ozone concentration: 1.5 times ambient ozone concentration     

  • Ozone exposure: Whole experimental period

  • Sampling time: End of exposure period

  • Platform: Microarray

  • Year of study: 2005

  • Location: USA

Title: Gene expression patterns of trembling aspen trees following long-term exposure to interacting elevated CO2 and tropospheric O3

 

Summary: Expression of 4600 poplar expressed sequence tags (ESTs) was studied over the 2001–2002 growing seasons using trees of the moderately ozone (O3)-tolerant trembling aspen (Populus tremuloide) clone 216 exposed to elevated CO2 and/or O3 for their entire 5-yr life history. Based on replication of the experiment in years 2001 and 2002, 238 genes showed qualitatively similar expression in at least one treatment and were retained for analysis. Of these 238 genes, 185 were significantly regulated (1.5-fold) from one year to the other in at least one treatment studied. Less than 1% of the genes regulated 2-fold or more. In the elevated CO2 treatment, relatively small numbers of genes were upregulated, whereas in the O3 treatment, higher expression of many signaling and defense-related genes and lower expression of several photosynthesis and energy related genes were observed. Senescence-associated genes (SAGs) and genes involved in the flavanoid pathway were also up-regulated under O3, with or without CO2 treatment. Interestingly, the combined treatment of CO2 plus O3 resulted in the differential expression of genes that were not up-regulated with individual gas treatments. This study represents the first investigation into gene expression following long term exposure of trees to the interacting effects of elevated CO2 and O3 under field conditions. Patterns of gene-specific regulation described in this study correlated with previously published physiological responses of aspen clone 216.

 

Data repository: http://mycor.nancy.inra.fr/poplarDB/pub.html

Reference: Gupta, P., Duplessis, S., White, H., Karnosky, D.F., Martin, F. and Podila, G.K., 2005. Gene expression patterns of trembling aspen trees following long‐term exposure to interacting elevated CO2 and tropospheric O3. New Phytologist, 167(1), pp.129-142.

clone-ID
Accession n�
O3/C
O3/C-1
O3/C-2
O3
Gene function
R09B09
CA822509
1.24
1.22
1.23
0.03281
40s ribosomal protein s17-like
R28H05
CA823614
1.41
1.36
1.38
0.0012
allergen DAC g II
F05E03
CA820898
1.2
1.35
1.28
0.03675
aquaporin
F08B04
CA821074
1.1
1.15
1.12
0.02421
auxin-binding protein abp19
F06D12
CA820955
1.26
1.27
1.27
0.01949
chlorophyll a/b-binding protein cp24
F01H03
CV734645
1.2
1.22
1.21
0.00573
hypothetical protein
F05C10
CA820887
1.24
1.39
1.31
0.02502
hypothetical protein
R17A03
CA822958
1.22
1.48
1.35
0.04958
latex profilin hev b 8
F11C03
CA821164
1.41
1.43
1.42
0.00033
lipoamide dehydrogenase
R38H06
CA824250
1.28
1.5
1.39
0.03578
S-adenosylmethionine carboxylase
F05G11
CA820919
1.51
1.47
1.49
0.02791
wound-induced protein
R09B07
CA822507
1.39
1.42
1.4
0.04526
14-3-3 protein
R56D02
CA825277
1.53
1.36
1.44
0.02208
40S ribosomal protein
R17D02
CA822968
1.43
1.54
1.48
0.02544
40S ribosomal protein S19
R02B05
CA821964
1.54
1.43
1.48
0.00546
60S ribosomal protein L15
R06B09
CA822293
1.52
1.42
1.47
0.01147
actin depolymerizing factor 1
R06E05
CA822316
1.45
1.43
1.44
0.04012
calmodulin
R06H07
CA822343
1.29
1.52
1.41
0.03107
cytoplasmic fructose-bisphosphate aldolase
R09E10
CA822540
1.33
1.56
1.44
0.03182
hypothetical protein
R26G06
CA823476
1.56
1.4
1.48
0.01036
hypothetical protein
R01G12
CB833341
1.51
1.39
1.45
0.01382
mitochondrial ATP synthase, epsilon chain
R77F05
CA826402
1.04
1.19
1.12
0.03569
polyubiquitin
R34G09
CA824005
1.29
1.49
1.39
0.04154
ribosomal protein L3
R27E10
CA823524
1.46
1.38
1.42
0.00352
S-adenosylmethionine synthetase
R01C02
CA821931
1.34
1.4
1.37
0.00571
60s ribosomal protein L15
R39G12
CA824311
1.32
1.32
1.32
0.02089
aquaporin 2
R58C11
CA825403
1.31
1.29
1.3
0.00711
catalase
R32D08
CA823854
1.31
1.62
1.47
0.04541
chalcone-flavonone isomerase b
F11A09
CA821136
1.27
1.57
1.42
0.04586
histone H3
F06C11
CA820947
1.31
1.36
1.33
0.0093
hypothetical protein
R04D04
CA822153
1.47
1.26
1.36
0.03504
hypothetical protein
R18C02
CA822991
1.34
1.53
1.43
0.01785
hypothetical protein
R01G07
CB833336
1.52
1.43
1.48
0.00965
mitochondrial ATP synthase, gamma chain
R02H11
CA822037
1.26
1.33
1.29
0.02997
peptidyl-prolyl cis-trans isomerase
F07D03
CA821017
1.33
1.24
1.29
0.04925
protein phosphatase
F06G07
CA820971
1.63
1.31
1.47
0.04452
SOUL-like protein
R26B04
CA823435
1.42
1.47
1.45
0.00666
specific tissue protein 2
R32D09
CA823855
1.2
1.3
1.25
0.03249
water stress-induced aquaporin MIPrwc-3
F03C08
CA820786
1.41
1.57
1.49
0.01889
hypothetical protein
R04C10
CA822148
2.25
1.64
1.94
0.04808
calmodulin 1
R04E08
CA822166
2.04
1.91
1.97
0.00161
histone H4
R06A09
CA822285
1.51
1.85
1.68
0.03689
ADP-ribosylation factor 1
R08B12
CA822430
1.73
2
1.86
0.00712
zinc-finger protein 1
R12E11
CA822712
1.92
1.9
1.91
0.04547
sucrose synthase
R14F06
CA822846
1.67
1.66
1.66
0.04799
histone H2b
R14F07
CA822847
1.78
1.35
1.57
0.04803
steroid sulfotransferase 3
R16F04
CA822942
1.81
1.6
1.71
0.00723
ribosomal protein S25
R16G02
CA822945
1.75
1.51
1.63
0.01647
alpha-6 tubulin
R16G03
CA822946
1.38
1.49
1.44
0.01327
xyloglucan endotransglycosylase
R18B06
CA822984
2.07
1.69
1.88
0.04904
cysteine protease
R21B12
CA823125
1.78
1.85
1.81
0.0033
ATP-citrate lyase
R26A01
CA823424
1.55
1.9
1.73
0.02088
ribosomal protein L8
R27F12
CA823535
1.75
1.7
1.73
0.01266
histone H4
R27H05
CA823548
1.61
2.14
1.87
0.02579
ubiquitin/ribosomal fusion protein S27a
R29G12
CA823688
1.93
1.68
1.8
0.01116
histone H4
R32G08
CA823885
1.47
1.83
1.65
0.03123
hypothetical protein
R33H09
CA823946
1.64
1.69
1.67
0.0152
histone H4
R34G08
CA824004
1.91
1.65
1.78
0.01398
histone H3
R36E07
CA824103
1.81
1.74
1.78
0.02528
calmodulin 1
R38G07
CA824244
2.01
1.26
1.63
hypothetical protein
R77F05
CA826402
1.57
2.4
1.99
polyubiquitin
F02B07
CA820728
2.1
1.72
1.91
0.01207
wound-induced protein
F06H08
CA820981
1.75
2.87
2.31
0.04486
hypothetical protein
R06C05
CA822298
1.9
1.77
1.84
0.00295
histone H3
R10A04
CA822570
2.64
2.5
2.57
0.0338
wound-induced protein
R20E04
CA823085
1.74
1.56
1.65
0.04973
beta tubulin 1
R25D04
CA823386
2.08
2.08
2.08
0.00435
wound-induced protein
R33H02
CA823880
2.1
1.59
1.84
0.026
histone H4
R41F12
CA824408
1.9
1.27
1.58
pop3 wound-induced protein
R44H01
CA824581
1.5
1.61
1.55
0.00431
tubulin alpha-2/alpha-4 chain
R46C01
CA824657
1.73
1.63
1.68
0.0078
pop3 wound-induced protein
R57F07
CA825362
1.18
1.76
1.47
naringenin-chalcone synthase
R71B12
CA826016
1.54
1.77
1.65
0.01343
leucoanthocyanidin dioxygenase
R71C11
CA826023
2.04
1.32
1.68
hypothetical protein
R75A10
CA826245
1.79
1.55
1.67
0.01818
polyubiquitin
F11C01
CA821162
-1.11
-1.17
-1.14
0.02883
eukaryotic translation initiation factor 2 alpha
F00D02
CV734581
-1.28
-1.38
-1.33
0.01164
phytochrome-associated protein 1
F01E06
CV734620
-1.13
-1.08
-1.1
0.03985
plastocyanin b
F06B09
CA820941
-1.44
-1.36
-1.4
0.01777
rubisco small subunit
F07C11
CA821013
-1.41
-1.36
-1.39
0.00147
rubisco small subunit 1
F07G10
CA821045
1.13
1.09
1.11
0.01469
auxin-binding protein abp19
R38G10
CA824246
1.36
1.24
1.3
0.0228
fructose bisphosphate aldolase
F04G09
CA820855
1.3
1.15
1.23
0.04675
hypothetical protein
R10E04
CA822599
1.3
1.29
1.3
0.04658
plastidic fructose-bisphosphate aldolase
R09H05
CA822563
1.19
1.18
1.19
0.01009
thioredoxin
R26D09
CA823454
1.44
1.54
1.49
0.01407
hypothetical protein
F07E11
CA821031
1.39
1.5
1.45
0.02697
plasma membrane intrinsic protein 1
R77F05
CA826402
1.29
1.44
1.36
0.03043
polyubiquitin
F05G02
CA820913
1.21
1.25
1.23
0.04353
PSI reaction centre subunit
R21D03
CA823137
1.24
1.96
1.6
40S ribosomal protein S11
R22D07
CA823207
1.7
1.95
1.82
0.00762
ribosomal protein L8
R23A07
CA823248
1.6
1.67
1.64
0.00657
40S ribosomal protein S25
R25A01
CA823357
1.53
1.56
1.54
0.00166
profilin
R25F07
CA823402
2.02
1.6
1.81
0.02828
inorganic pyrophosphatase
R26D03
CA823451
1.4
1.6
1.5
0.01414
histone H2a
R26G01
CA823474
1.64
1.77
1.7
0.00397
ribosomal protein S20
R27F07
CA823531
1.47
1.84
1.65
0.0449
Bax-induced cell death inhibitor 1
R31E05
CA823796
1.46
1.8
1.63
0.04649
40S ribosomal protein S25
R34B02
CA823960
1.52
1.76
1.64
0.01261
ubiquitin-conjugating enzyme E2-17 kda
R40D06
CA824343
1.54
1.56
1.55
0.00479
peptidyl-prolyl cis-trans isomerase
R70A09
CA825940
1.62
1.68
1.65
0.02808
elongation factor 1-alpha
F09A05
CA821085
1.4
1.8
1.6
0.04025
60s acidic ribosomal protein po
F11A21
CA821144
1.74
1.65
1.69
0.00783
wound-induced protein
R02G08
CA822022
1.53
1.56
1.55
0.00026
60S ribosomal protein L41
R02H12
CA822038
1.82
1.39
1.61
0.04149
MAP3K-like protein kinase
R05H09
CA822200
1.44
1.72
1.58
0.02328
hypothetical protein
R07E06
CA822383
1.87
1.46
1.67
0.03829
wound-induced protein
R09A11
CA822499
1.17
1.76
1.47
polyubiquitin
R19B06
CB833358
1.61
2.03
1.82
0.01906
hypothetical protein
R20A02
CA823050
1.62
1.34
1.48
elongation factor eF-2
R23E11
CA823289
1.45
1.7
1.57
0.01721
elongation factor 1b gamma
R25D07
CA823389
1.6
1.17
1.38
S-adenosylmethionine synthetase
R25G01
CA823406
1.52
1.18
1.35
S-adenosylmethionine synthetase
R36D06
CA824094
1.49
1.51
1.5
0.02999
actin-depolymerizing factor 2
R37G03
CA824181
1.77
1.38
1.57
histone H2b
R41C03
CA824387
1.75
1.51
1.63
0.01591
60S ribosomal protein L8
R52A02
CA825012
1.31
1.27
1.29
0.0027
histone H3.2
R52H11
CA825068
1.49
1.57
1.53
0.01474
ubiquitin S5
R69H11
CA825933
1.9
1.31
1.6
hypothetical protein
F04C06
CA820835
1.27
1.41
1.34
0.01461
pop3 wound-induced protein
R01C12
CB833317
1.43
1.79
1.61
0.03045
40S ribosomal protein S7
R13C11
CA822755
1.49
1.73
1.61
0.03104
chalcone-flavonone isomerase
R15A03
CA822870
2.03
1.46
1.74
0.04622
hypothetical protein
R17C05
CA822967
1.75
1.7
1.72
0.02518
hypothetical protein
R19H04
CB833383
1.83
1.74
1.79
0.00107
hypothetical protein
R25C01
CA823375
1.59
1.53
1.56
0.01048
60S ribosomal protein L13
R45H09
CA824638
1.86
1.47
1.66
metallothionein 1b
R46G02
CA824693
1.32
1.43
1.38
hypothetical protein
R54C11
CA825157
1.42
1.41
1.42
0.00283
hypothetical protein
R57C09
CA825341
1.59
1.26
1.43
mitochondrial phosphate transporter
R71C08
CA826021
1.87
1.23
1.55
aquaporin
R73E09
CA826154
1.52
1.38
1.45
dehydrin
R75C04
CA826255
1.57
1.42
1.49
0.01327
glyceraldehyde-3-phosphate dehydrogenase
R75F07
CA826280
1.33
1.21
1.27
0.02943
1-aminocyclopropane-1-carboxylate oxidase
R77F06
CA826403
1.91
2.08
1.99
formate dehydrogenase
F01B02
CV734598
1.94
2.02
1.98
0.00144
wound-induced protein
F02G09
CA820757
2.04
1.13
1.59
hypothetical protein
F11B12
CA821155
1.08
2.17
1.63
thaumatin-like protein
R03B06
CA822054
1.37
1.68
1.53
histone H3
R55C09
CA825218
1.53
1.51
1.52
0.04923
actin-depolymerizing factor 2
R02B06
CA821965
1.51
1.39
1.45
0.02913
hypothetical protein
R02C10
CA821980
1.42
1.38
1.4
0.04534
enolase 2 (2-phosphoglycerate dehydratase 2)
R02G04
CA822019
1.27
1.48
1.37
actin depolymerizing factor 1
R04A10
CA822125
1.22
1.45
1.34
peptidyl-prolyl cis-trans isomerase
R19G01
CB833378
1.52
1.6
1.56
0.01047
translation initiation factor 5a
R19H01
CB833381
1.85
1.18
1.51
actin-depolymerizing factor 2
R19H07
CB833384
1.81
1.22
1.51
metallothionein 1b
R34C03
CA823971
1.59
1.43
1.51
0.02105
ubiquitin extension protein
R34H09
CA824013
1.55
1.38
1.46
0.01947
ribosomal protein S27
R36G06
CA824118
1.57
1.28
1.43
0.04154
actin-depolymerizing factor 2
R37F06
CA824173
1.49
1.45
1.47
0.01777
mitochondrial ATPase beta subunit
R46F07
CA824687
1.55
1.12
1.33
calmodulin CAM1
R46F09
CA824689
1.38
1.42
1.4
0.00355
polyubiquitin
F05H11
CA820926
2.09
1.4
1.74
cysteine protease
F06G11
CA820974
1.45
1.52
1.48
kinetochore skp1 protein
F07B12
CA821005
2.77
1.19
1.98
glutamine synthetase
F08A03
CA821062
1.38
1.14
1.26
oxygen evolving enhancer protein 1
F11D23
CA821201
1.52
1.62
1.57
0.04621
ATP-dependent Clp protease
R01B09
CB833311
-1.09
1.32
1.13
histone H3.2
R02H08
CA822034
1.47
1.91
1.69
0.04107
glutamine synthetase
R02H11
CA822037
1.59
1.41
1.5
0.0162
peptidyl-prolyl cis-trans isomerase
R03D06
CA822075
1.55
2.02
1.78
0.02634
alcohol dehydrogenase
R10G01
CA822614
1.98
1.61
1.8
0.01635
fimbriata/kinetochore-associated protein Skp1
R13H06
CA822790
1.58
1.56
1.57
0.00892
ubiquitin-conjugating protein
R18D08
CA823001
1.81
1.63
1.72
0.00506
hypothetical protein
R21A03
CA823109
-1.04
1.51
1.24
cinnamate 4-hydroxylase
R22H10
CA823242
1.37
1.85
1.61
60S ribosomal protein l7a
R23F06
CA823296
1.38
1.74
1.56
0.04632
ribosomal protein L8
R26E10
CA823465
2.78
1.52
2.15
metallothionein 2a
R29H04
CA823692
1.4
1.44
1.42
0.03948
ribosomal protein L8
R74G09
CA826232
-1.03
1.16
1.07
aquaporin
F06A09
CA820932
2.03
1.51
1.77
hypothetical protein
F07G07
CA821043
1.7
1.64
1.67
0.00096
wound-induced protein
F11C02
CA821163
1.6
2
1.8
0.03842
60S acidic ribosomal protein P1 (L12)
F11D05
CA821187
1.72
1.54
1.63
0.0127
hypothetical protein
R00A04
CB833299
1.15
1.87
1.51
cytosolic glyceraldehyde 3-phosphate dehydrogenase
R02B04
CA821963
1.49
1.68
1.59
0.04745
60S ribosomal protein L7a
R02C05
CA821976
1.49
1.9
1.69
0.03178
cytochome b5
R02F10
CA822013
1.73
1.46
1.6
0.03676
methionine adenosyltransferase
R03F04
CA822093
1.55
1.78
1.66
0.0115
14-3-3-like protein
R05H08
CA822199
2.3
1.04
1.67
metallothionein 1a
R06A01
CA822280
1.66
1.75
1.71
0.03116
S-adenosylmethionine synthetase
R08D02
CA822443
2.38
1.24
1.81
metallothionein 1b
R09G11
CA822559
1.39
1.89
1.64
wound-induced protein
R11A06
CA822628
1.79
1.41
1.6
0.03321
translation initiation factor 5a
R13G03
CA822781
1.55
1.68
1.62
0.02096
60S ribosomal protein L9 (gibberellin-regulated protein ga)
R14H10
CA822867
1.87
1.52
1.7
0.01911
ubiquitin c
R15H06
CA822913
1.6
2.11
1.86
0.02977
hypothetical protein
R20A07
CA823054
1.44
1.89
1.67
0.0376
H+-transporting ATPase
(2001)
(2002)
(mean)
lnP-value � 0.05
F00D03
CV734582
-1.6
-1.33
-1.45
0.03336
rubisco small subunit
F01B11
CV734604
-1.65
-1.57
-1.61
0.00263
rubisco small subunit
F01C04
CV734608
-1.68
-1.96
-1.81
0.01519
rubisco small subunit
F01E12
CV734624
-1.41
-1.57
-1.49
0.04605
auxin-binding protein abp19
F01H07
CV734649
-2.13
-1.81
-1.95
0.01106
rubisco small subunit
F02E05
CA820743
-1.74
-1.83
-1.78
0.00123
rubisco small subunit
F02F08
CA820750
1.35
-1.96
-1.08
carbonate dehydratase
F03A04
CA820769
-1.66
-1.22
-1.4
rubisco small subunit
F05D07
CA820891
-1.67
-2.19
-1.89
0.02421
rubisco small subunit
F06E12
CA820962
1.02
-1.14
-1.06
ATP synthase gamma chain, chloroplast