Species & Dataset

  • Pisum sativum

  • Common name: Garden pea

  • Family: Fabaceae

Foliar Ozone Injury

pisum injury.jpg

Experiment

  • Cultivar: Pisum sativum L.

  • Tissue: Fifth leaf from the top

  • Ozone concentration: Ambient ozone (12.5 nL L−1 ± 0.96 nL L−1)

  • Elevated ozone (151.2 nL L−1 ± 0.72 nL L−1)

  • Ozone exposure: Throughout the experiment (8 hours/day)

  • Sampling time: 11-13 days after planting

  • Platform: Hiseq 2000 (Illumina)

  • Year of study: 2015

  • Location: Urbana, USA

Title: A comparative analysis of transcriptomic, biochemical, and physiological responses to elevated ozone identifies species-specific mechanisms of resilience in legume crops

 

Summary: Current concentrations of tropospheric ozone ([O3]) pollution negatively impact plant metabolism, which can result in decreased crop yields. Interspecific variation in the physiological response of plants to elevated [O3] exists; however, the underlying cellular responses explaining species-specific differences are largely unknown. Here, a physiological screen has been performed on multiple varieties of legume species. Three varieties of garden pea (Pisum sativum L.) were resilient to elevated [O3]. Garden pea showed no change in photosynthetic capacity or leaf longevity when exposed to elevated [O3], in contrast to varieties of soybean (Glycine max (L.) Merr.) and common bean (Phaseolus vulgaris L.). Global transcriptomic and targeted biochemical analyses were then done to examine the mechanistic differences in legume responses to elevated [O3]. In all three species, there was an O3-mediated reduction in specific leaf weight and total non-structural carbohydrate content, as well as increased abundance of respiration-related transcripts. Differences specific to garden pea included a pronounced increase in the abundance of GLUTATHIONE REDUCTASE transcript, as well as greater contents of foliar glutathione, apoplastic ascorbate, and sucrose in elevated [O3]. These results suggest that garden pea may have had greater capacity for detoxification, which prevented net losses in CO2 fixation in an elevated [O3] environment.

 

Data repository: NCBI [GenBank: SRP009826]

Reference: Yendrek, C.R., Koester, R.P. and Ainsworth, E.A., 2015. A comparative analysis of transcriptomic, biochemical, and physiological responses to elevated ozone identifies species-specific mechanisms of resilience in legume crops. Journal of experimental botany, 66(22), pp.7101-7112.

Gene id
log2FC
Ambient RPKM
Elevated RPKM
Gene Annotation
ID_Pisum_sativum_v2_Contig4373
0.52
90.88
126.78
ACONITASE
ID_Pisum_sativum_v2_Contig3045
1.01
3.64
7.18
ISOCITRATE DEHYDROGENASE
ID296188_p.sativum_wa1_contig11295
0.67
18.12
28.21
ISOCITRATE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig5799
0.59
79.53
116.77
SUCCINYL-CoA LIGASE
ID_Pisum_sativum_v2_Contig238
-0.81
4.84
2.68
MALATE DEHYDROGENASE
ID284036_p.sativum_wa1_contig18557
2.01
38.95
154.13
MALATE OXIDOREDUCTASE
ID_Pisum_sativum_v2_Contig4372
0.85
80.66
141.09
MALATE OXIDOREDUCTASE
ID_Pisum_sativum_v2_Contig1835
0.61
45.57
67.83
ATP-CITRATE LYASE
ID298794_p.sativum_wa1_contig09721
0.82
6.59
11.35
CARBONIC ANHYDRASE
ID_Pisum_sativum_v2_Contig7774
2.43
23.06
121.22
NADH-DEHYDROGENASE [complex I]
ID271967_p.sativum_wa1_contig23606
-1.32
3.62
1.38
NADH-DEHYDROGENASE [complex I]
ID266822_p.sativum_wa1_contig10263
4.31
0.23
4.33
NADH-DEHYDROGENASE [complex I]
ID_Pisum_sativum_v2_Contig2147
-0.69
167.66
99.88
NADH-DEHYDROGENASE [complex I, carbonic anhydrase]
ID266675_p.sativum_wa1_contig28876
4.1
15.49
259.81
NADH-DEHYDROGENASE [localization unclear]
ID_Pisum_sativum_v2_Contig3718
-0.82
8.62
4.74
NADH-DEHYDROGENASE [localization unclear]
ID_Pisum_sativum_v2_Contig4589
1.45
53.24
141.15
NADH-DEHYDROGENASE [type II, external]
ID_Pisum_sativum_v2_Contig3315
3.31
6.31
61.72
NADH-DEHYDROGENASE [type II, internal matrix]
ID_Pisum_sativum_v2_Contig8415
-0.75
91.46
52.42
NADH-DEHYDROGENASE [type II, internal matrix]
ID270489_p.sativum_wa1_contig25191
1.24
29.88
68.02
ALTERNATIVE OXIDASE
ID_Pisum_sativum_v2_Contig2718
2.52
3.53
19.75
ALTERNATIVE OXIDASE
ID272230_p.sativum_wa1_contig10126
2.7
2.97
19.04
ALTERNATIVE OXIDASE
ID281263_p.sativum_wa1_contig28984
1.5
5.92
16.4
CYTOCHROME C
ID281290_p.sativum_wa1_contig30839
0.66
105.88
163.37
CYTOCHROME C
ID_Pisum_sativum_v2_Contig7865
-0.71
65.61
38.72
CYTOCHROME C
ID297249_p.sativum_wa1_contig34819
-0.69
54.83
32.57
CYTOCHROME C
ID17056_EX570908
0.61
16.64
24.71
CYTOCHROME C
ID263334_p.sativum_wa1_contig08262
1.02
6.34
12.48
CYTOCHROME C
ID_Pisum_sativum_v2_Contig2148
1.03
6.81
13.51
CYTOCHROME C OXIDASE
ID274179_p.sativum_wa1_contig22645
2.33
11.82
58.61
Fermentation: LACTATE DEHYDROGENASE
ID270381_p.sativum_wa1_contig10911
-1.25
4.71
1.88
Fermentation: ALDEHYDE DEHYDROGENASE
ID266021_p.sativum_wa1_contig20179
4.35
9.84
194.33
Fermentation: ALDEHYDE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig5230
-1.19
83.88
35.24
Fermentation: ALDEHYDE DEHYDROGENASE
ID295295_p.sativum_wa1_contig08348
1
3.07
6.06
Fermentation: ALDEHYDE DEHYDROGENASE
ID263664_p.sativum_wa1_contig13195
-0.63
275.08
170.77
Gluconeogenesis / glyoxylate cycle: MALATE DEHYDROGENASE
ID268005_p.sativum_wa1_contig11567
-1.1
3.28
1.51
Pentose phosphate pathway, oxidative: GLUCOSE 6-PHOSPHATE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig3116
-0.55
38.49
25.48
Pentose phosphate pathway, oxidative: GLUCOSE 6-PHOSPHATE DEHYDROGENASE
ID262852_p.sativum_wa1_contig18926
1.91
23.28
85.77
Pentose phosphate pathway, oxidative: GLUCOSE 6-PHOSPHATE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig5003
0.68
145.35
228.24
Pentose phosphate pathway, oxidative: 6-PHOSPHOGLUCONATE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig1969
-0.73
101.73
58.9
Pentose phosphate pathway, non-reductive: RIBOSE 5-PHOSPHATE ISOMERASE
ID299182_p.sativum_wa1_contig21283
0.5
84.46
115.44
Pentose phosphate pathway, non-reductive: RIBOSE 5-PHOSPHATE ISOMERASE
ID292516_p.sativum_wa1_contig29219
-0.57
59.26
38.27
Pentose phosphate pathway: electron transfer
ID296830_p.sativum_wa1_contig18841
5.45
1.99
84.39
ASCORBATE OXIDASE [cell wall]
ID_Pisum_sativum_v2_Contig4699
-0.12
145.03
129.09
ASCORBATE OXIDASE [cell wall]
ID270485_p.sativum_wa1_contig24721
-0.09
726.26
661.1
ASCORBATE PEROXIDASE 1 [cytoplasm]
ID290629_p.sativum_wa1_contig26708
-0.27
8.97
7.21
ASCORBATE PEROXIDASE 3 [microsome]
ID284080_p.sativum_wa1_contig21676
-0.28
187.4
149.09
ASCORBATE PEROXIDASE 3 [microsome]
ID_Pisum_sativum_v2_Contig5697
0.01
154.5
150.41
ASCORBATE PEROXIDASE [thylakoid]
ID_Pisum_sativum_v2_Contig4094
-0.63
1378.58
853.61
CATALASE 2
ID_Pisum_sativum_v2_Contig1642
0.57
1.81
2.61
DEHYDROASCORBATE REDUCTASE 2
ID_Pisum_sativum_v2_Contig8801
-0.42
3.83
2.77
DEHYDROASCORBATE REDUCTASE 3 [chloroplast]
ID262802_p.sativum_wa1_contig12414
0.01
24.6
23.83
DEHYDROASCORBATE REDUCTASE 3 [chloroplast]
ID281337_p.sativum_wa1_contig33760
0.07
7.52
7.59
GLUTATHIONE PEROXIDASE 3
ID263199_p.sativum_wa1_contig27565
0.28
2.89
3.45
GLUTATHIONE PEROXIDASE 3
ID_Pisum_sativum_v2_Contig7800
-0.45
66.45
46.99
GLUTATHIONE PEROXIDASE 4
ID_Pisum_sativum_v2_Contig1327
0.19
7.15
7.95
GLUTATHIONE PEROXIDASE 6
ID270618_p.sativum_wa1_contig03738
-0.07
184.69
170.34
GLUTATHIONE PEROXIDASE 7
ID_Pisum_sativum_v2_Contig8768
1.23
97.6
224.23
GLUTATHIONE REDUCTASE [cytoplasm]
ID_Pisum_sativum_v2_Contig4628
-0.2
153.54
129.6
GLUTATHIONE REDUCTASE [chloroplast]
ID_Pisum_sativum_v2_Contig4113
-0.08
354.43
325.58
MONODEHYDROASCORBATE REDUCTASE 1
ID289338_p.sativum_wa1_contig19560
-0.36
112.92
84.57
MONODEHYDROASCORBATE REDUCTASE 4
ID_Pisum_sativum_v2_Contig4052
-0.24
82.37
67.2
SUPEROXIDE DISMUTASE 3 [Cu,Zn]
ID_Pisum_sativum_v2_Contig4187
-0.04
104.8
99.06
SUPEROXIDE DISMUTASE 1 [Mn]
ID291601_p.sativum_wa1_contig26572
4.74
1.3
34.8
?-GLUTAMYL TRANSPEPTIDASE
ID278435_p.sativum_wa1_contig34236
5.21
0.65
23.87
?-GLUTAMYL TRANSPEPTIDASE
ID_Pisum_sativum_v2_Contig4841
-0.03
96.07
91.74
GLUTAMATE-CYSTEINE LIGASE
ID286494_p.sativum_wa1_contig25210
-0.29
62.68
57.01
GLUTATHIONE SYNTHETASE
ID295758_p.sativum_wa1_contig30872
-0.24
56.78
46.4
GLUTATHIONE SYNTHETASE
ID_Pisum_sativum_v2_Contig36
-0.57
53.72
34.8
GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE [cytoplasm]
ID_Pisum_sativum_v2_Contig6409
0.83
195.2
339.4
GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE [cytoplasm]
ID282363_p.sativum_wa1_contig24234
-0.76
89.06
50.53
PHOSPHOGLYCERATE MUTASE [cytoplasm]
ID290074_p.sativum_wa1_contig28839
0.93
11.97
22.15
PHOSPHOGLYCERATE MUTASE [cytoplasm]
ID282982_p.sativum_wa1_contig26055
1.61
7.51
22.31
PHOSPHOGLYCERATE MUTASE [cytoplasm]
ID286398_p.sativum_wa1_contig19099
1.53
12.42
34.93
ENOLASE [cytoplasm]
ID_Pisum_sativum_v2_Contig4678
0.51
109.52
152.14
PHOSPHO-ENOL-PYRUVATE CARBOXYLASE [cytoplasm]
ID297143_p.sativum_wa1_contig21264
1.2
57.72
129.36
PHOSPHO-ENOL-PYRUVATE CARBOXYLASE [cytoplasm]
ID14082_GH719589
1.7
20.91
66.2
PHOSPHO-ENOL-PYRUVATE CARBOXYLASE [cytoplasm]
ID_Pisum_sativum_v2_Contig7139
1.38
42.1
106.52
PHOSPHO-ENOL-PYRUVATE CARBOXYLASE [cytoplasm]
ID296901_p.sativum_wa1_contig28437
1.47
6.4
17.29
PHOSPHOFRUCTOKINASE [plastid]
ID_Pisum_sativum_v2_Contig6823
1.26
9.86
23.11
PHOSPHOFRUCTOKINASE [plastid]
ID265166_p.sativum_wa1_contig23674
-0.53
71.57
47.98
PYRUVATE KINASE [plastid]
ID284266_p.sativum_wa1_contig33550
-0.52
70.21
46.98
PYRUVATE KINASE [plastid]
ID_Pisum_sativum_v2_Contig2999
0.59
37.28
54.82
PYRUVATE KINASE [plastid]
ID277035_p.sativum_wa1_contig18485
-0.81
12.15
6.69
PYRUVATE KINASE [plastid]
ID272847_p.sativum_wa1_contig12510
-0.8
8.7
4.83
PYRUVATE KINASE [plastid]
ID_Pisum_sativum_v2_Contig4652
-0.5
79.79
54.56
PYRUVATE KINASE [plastid]
ID282363_p.sativum_wa1_contig24234
-0.76
89.06
50.53
PHOSPHOGLYCERATE MUTASE [localization unclear]
ID290074_p.sativum_wa1_contig28839
0.93
11.97
22.15
PHOSPHOGLYCERATE MUTASE [localization unclear]
ID286427_p.sativum_wa1_contig20903
2.14
41.97
180.49
PHOSPHOGLYCERATE MUTASE [localization unclear]
ID282982_p.sativum_wa1_contig26055
1.61
7.51
22.31
PHOSPHOGLYCERATE MUTASE [localization unclear]
ID278763_p.sativum_wa1_contig17872
0.97
37.46
70.48
PYRUVATE DEHYDROGENASE [E2]
ID_Pisum_sativum_v2_Contig4865
0.73
71.97
116.75
CITRATE SYNTHASE