Species & Dataset

Experiment

Foliar Ozone Injury

  • Pisum sativum

  • Common name: Garden pea

  • Family: Fabaceae

  • Cultivar: Pisum sativum L.

  • Tissue: Fifth leaf from the top

  • Ozone concentration: Ambient ozone (12.5 nL L−1 ± 0.96 nL L−1)

  • Elevated ozone (151.2 nL L−1 ± 0.72 nL L−1)

  • Ozone exposure: Throughout the experiment (8 hours/day)

  • Sampling time: 11-13 days after planting

  • Platform: Hiseq 2000 (Illumina)

  • Year of study: 2015

  • Location: Urbana, USA

pisum injury.jpg

Title: A comparative analysis of transcriptomic, biochemical, and physiological responses to elevated ozone identifies species-specific mechanisms of resilience in legume crops

 

Summary: Current concentrations of tropospheric ozone ([O3]) pollution negatively impact plant metabolism, which can result in decreased crop yields. Interspecific variation in the physiological response of plants to elevated [O3] exists; however, the underlying cellular responses explaining species-specific differences are largely unknown. Here, a physiological screen has been performed on multiple varieties of legume species. Three varieties of garden pea (Pisum sativum L.) were resilient to elevated [O3]. Garden pea showed no change in photosynthetic capacity or leaf longevity when exposed to elevated [O3], in contrast to varieties of soybean (Glycine max (L.) Merr.) and common bean (Phaseolus vulgaris L.). Global transcriptomic and targeted biochemical analyses were then done to examine the mechanistic differences in legume responses to elevated [O3]. In all three species, there was an O3-mediated reduction in specific leaf weight and total non-structural carbohydrate content, as well as increased abundance of respiration-related transcripts. Differences specific to garden pea included a pronounced increase in the abundance of GLUTATHIONE REDUCTASE transcript, as well as greater contents of foliar glutathione, apoplastic ascorbate, and sucrose in elevated [O3]. These results suggest that garden pea may have had greater capacity for detoxification, which prevented net losses in CO2 fixation in an elevated [O3] environment.

 

Data repository: NCBI [GenBank: SRP009826]

Reference: Yendrek, C.R., Koester, R.P. and Ainsworth, E.A., 2015. A comparative analysis of transcriptomic, biochemical, and physiological responses to elevated ozone identifies species-specific mechanisms of resilience in legume crops. Journal of experimental botany, 66(22), pp.7101-7112.

Gene id
AGI Gene Code
Uniprot ID
Bin Code
Bin Name
log2FC
Functional Annotation
ID_Pisum_sativum_v2_Contig4373
AT4G35830
Q42560
8.1.3
TCA / org transformation.TCA.aconitase
0.52
ACONITASE
ID_Pisum_sativum_v2_Contig3045
AT1G65930
Q9SRZ6
8.1.4
TCA / org transformation.TCA.IDH
1.01
ISOCITRATE DEHYDROGENASE
ID296188_p.sativum_wa1_contig11295
AT1G65930
Q9SRZ6
8.1.4
TCA / org transformation.TCA.IDH
0.67
ISOCITRATE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig5799
AT5G23250
Q8LAD2
8.1.6
TCA / org transformation.TCA.succinyl-CoA ligase
0.59
SUCCINYL-CoA LIGASE
ID_Pisum_sativum_v2_Contig238
AT1G04410
P93819
8.2.9
TCA / org transformation.other organic acid transformatons.cyt MDH
-0.81
MALATE DEHYDROGENASE
ID284036_p.sativum_wa1_contig18557
AT5G25880
Q9XGZ0
8.2.10
TCA / org transformation.other organic acid transformatons.malic
2.01
MALATE OXIDOREDUCTASE
ID_Pisum_sativum_v2_Contig4372
AT5G11670
Q9LYG3
8.2.10
TCA / org transformation.other organic acid transformatons.malic
0.85
MALATE OXIDOREDUCTASE
ID_Pisum_sativum_v2_Contig1835
AT3G06650
Q9C522
8.2.11
TCA / org transformation.other organic acid transformatons.atp-citrate lyase
0.61
ATP-CITRATE LYASE
ID298794_p.sativum_wa1_contig09721
AT5G14740
P42737
8.3
TCA / org transformation.carbonic anhydrases
0.82
CARBONIC ANHYDRASE
ID_Pisum_sativum_v2_Contig7774
AT3G27890
Q9LK88
9.1.1
mitochondrial electron transport / ATP synthesis.NADH-DH.complex I
2.43
NADH-DEHYDROGENASE [complex I]
ID271967_p.sativum_wa1_contig23606
AT4G28510
O49460
9.1.1
mitochondrial electron transport / ATP synthesis.NADH-DH.complex I
-1.32
NADH-DEHYDROGENASE [complex I]
ID266822_p.sativum_wa1_contig10263
AT3G27890
Q9LK88
9.1.1
mitochondrial electron transport / ATP synthesis.NADH-DH.complex I
4.31
NADH-DEHYDROGENASE [complex I]
ID_Pisum_sativum_v2_Contig2147
AT1G74730
Q94F10
35.2
not assigned.unknown
-0.69
NADH-DEHYDROGENASE [complex I, carbonic anhydrase]
ID266675_p.sativum_wa1_contig28876
AT4G29330
Q8VZU9
29.5
protein.degradation
4.1
NADH-DEHYDROGENASE [localization unclear]
ID_Pisum_sativum_v2_Contig3718
ATMG00980
P92532
29.2.1.1.2.2.2
protein.synthesis.ribosomal protein.prokaryotic.mitochondrion.50S subunit.L2
-0.82
NADH-DEHYDROGENASE [localization unclear]
ID_Pisum_sativum_v2_Contig4589
AT4G05020
Q94BV7
9.2.1.2
mitochondrial electron transport / ATP synthesis.NADH-DH.type II.external
1.45
NADH-DEHYDROGENASE [type II, external]
ID_Pisum_sativum_v2_Contig3315
AT2G29990
O80874
9.2.1.4
mitochondrial electron transport / ATP synthesis.NADH-DH.type II.internal matrix
3.31
NADH-DEHYDROGENASE [type II, internal matrix]
ID_Pisum_sativum_v2_Contig8415
AT1G07180
Q8GWA1
9.2.1.4
mitochondrial electron transport / ATP synthesis.NADH-DH.type II.internal matrix
-0.75
NADH-DEHYDROGENASE [type II, internal matrix]
ID270489_p.sativum_wa1_contig25191
AT3G22370
Q39219
9.4
mitochondrial electron transport / ATP synthesis.alternative oxidase
1.24
ALTERNATIVE OXIDASE
ID_Pisum_sativum_v2_Contig2718
AT5G64210
O22049
9.4
mitochondrial electron transport / ATP synthesis.alternative oxidase
2.52
ALTERNATIVE OXIDASE
ID272230_p.sativum_wa1_contig10126
AT5G64210
O22049
9.4
mitochondrial electron transport / ATP synthesis.alternative oxidase
2.7
ALTERNATIVE OXIDASE
ID281263_p.sativum_wa1_contig28984
AT4G10040
Q9T0G2
9.6
mitochondrial electron transport / ATP synthesis.cytochrome c
1.5
CYTOCHROME C
ID281290_p.sativum_wa1_contig30839
AT4G10040
Q9T0G2
9.6
mitochondrial electron transport / ATP synthesis.cytochrome c
0.66
CYTOCHROME C
ID_Pisum_sativum_v2_Contig7865
AT1G49380
Q9XIA4
9.6
mitochondrial electron transport / ATP synthesis.cytochrome c
-0.71
CYTOCHROME C
ID297249_p.sativum_wa1_contig34819
AT1G49380
Q9XIA4
9.6
mitochondrial electron transport / ATP synthesis.cytochrome c
-0.69
CYTOCHROME C
ID17056_EX570908
#N/A
#N/A
#N/A
0.61
CYTOCHROME C
ID263334_p.sativum_wa1_contig08262
AT3G51790
Q96326
9.6
mitochondrial electron transport / ATP synthesis.cytochrome c
1.02
CYTOCHROME C
ID_Pisum_sativum_v2_Contig2148
AT1G80230
Q9SSB8
9.7
mitochondrial electron transport / ATP synthesis.cytochrome c oxidase
1.03
CYTOCHROME C OXIDASE
ID274179_p.sativum_wa1_contig22645
AT4G17260
O23569
5.1
fermentation.LDH
2.33
Fermentation: LACTATE DEHYDROGENASE
ID270381_p.sativum_wa1_contig10911
AT4G36250
Q70E96
5.10
fermentation.aldehyde dehydrogenase
-1.25
Fermentation: ALDEHYDE DEHYDROGENASE
ID266021_p.sativum_wa1_contig20179
AT1G44170
Q70DU8
5.10
fermentation.aldehyde dehydrogenase
4.35
Fermentation: ALDEHYDE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig5230
AT4G36250
Q70E96
5.10
fermentation.aldehyde dehydrogenase
-1.19
Fermentation: ALDEHYDE DEHYDROGENASE
ID295295_p.sativum_wa1_contig08348
AT1G23800
Q8S528
5.10
fermentation.aldehyde dehydrogenase
1
Fermentation: ALDEHYDE DEHYDROGENASE
ID263664_p.sativum_wa1_contig13195
AT5G09660
Q9ZP05
6.3
gluconeogenesis / glyoxylate cycle.Malate DH
-0.63
Gluconeogenesis / glyoxylate cycle: MALATE DEHYDROGENASE
ID268005_p.sativum_wa1_contig11567
AT1G24280
Q8L743
7.1.1
OPP.oxidative PP.G6PD
-1.1
Pentose phosphate pathway, oxidative: GLUCOSE 6-PHOSPHATE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig3116
AT5G13110
Q9FY99
7.1.1
OPP.oxidative PP.G6PD
-0.55
Pentose phosphate pathway, oxidative: GLUCOSE 6-PHOSPHATE DEHYDROGENASE
ID262852_p.sativum_wa1_contig18926
AT5G40760
Q9FJI5
7.1.1
OPP.oxidative PP.G6PD
1.91
Pentose phosphate pathway, oxidative: GLUCOSE 6-PHOSPHATE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig5003
AT3G02360
Q9FWA3
7.1.3
OPP.oxidative PP.6-phosphogluconate dehydrogenase
0.68
Pentose phosphate pathway, oxidative: 6-PHOSPHOGLUCONATE DEHYDROGENASE
ID_Pisum_sativum_v2_Contig1969
AT3G04790
Q9S726
1.3.10
PS.calvin cycle.Rib5P Isomerase
-0.73
Pentose phosphate pathway, non-reductive: RIBOSE 5-PHOSPHATE ISOMERASE
ID299182_p.sativum_wa1_contig21283
AT2G01290
Q9ZU38
35.2
not assigned.unknown
0.5
Pentose phosphate pathway, non-reductive: RIBOSE 5-PHOSPHATE ISOMERASE
ID292516_p.sativum_wa1_contig29219
AT4G14890
O23344
26.30
misc. other Ferredoxins and Rieske domain
-0.57
Pentose phosphate pathway: electron transfer
ID296830_p.sativum_wa1_contig18841
AT4G39830
A0A1P8B4N7
21.2.1
redox.ascorbate and glutathione.ascorbate
5.45
ASCORBATE OXIDASE [cell wall]
ID_Pisum_sativum_v2_Contig4699
AT5G21105
F4K6Z6
21.2.1
redox.ascorbate and glutathione.ascorbate
-0.12
ASCORBATE OXIDASE [cell wall]
ID270485_p.sativum_wa1_contig24721
AT1G07890
Q05431
21.2.1
redox.ascorbate and glutathione.ascorbate
-0.09
ASCORBATE PEROXIDASE 1 [cytoplasm]
ID290629_p.sativum_wa1_contig26708
AT4G35000
Q42564
21.2.1
redox.ascorbate and glutathione.ascorbate
-0.27
ASCORBATE PEROXIDASE 3 [microsome]
ID284080_p.sativum_wa1_contig21676
AT4G35000
Q42564
21.2.1
redox.ascorbate and glutathione.ascorbate
-0.28
ASCORBATE PEROXIDASE 3 [microsome]
ID_Pisum_sativum_v2_Contig5697
AT1G77490
Q42593
21.2.1
redox.ascorbate and glutathione.ascorbate
0.01
ASCORBATE PEROXIDASE [thylakoid]
ID_Pisum_sativum_v2_Contig4094
AT4G35090
P25819
21.6
redox.dismutases and catalases
-0.63
CATALASE 2
ID_Pisum_sativum_v2_Contig1642
AT1G75270
Q9FRL8
21.2.1
redox.ascorbate and glutathione.ascorbate
0.57
DEHYDROASCORBATE REDUCTASE 2
ID_Pisum_sativum_v2_Contig8801
AT5G16710
Q8LE52
21.2.1
redox.ascorbate and glutathione.ascorbate
-0.42
DEHYDROASCORBATE REDUCTASE 3 [chloroplast]
ID262802_p.sativum_wa1_contig12414
AT5G16710
Q8LE52
21.2.1
redox.ascorbate and glutathione.ascorbate
0.01
DEHYDROASCORBATE REDUCTASE 3 [chloroplast]
ID281337_p.sativum_wa1_contig33760
AT2G43350
O22850
21.2
redox.ascorbate and glutathione
0.07
GLUTATHIONE PEROXIDASE 3
ID263199_p.sativum_wa1_contig27565
AT2G43350
O22850
21.2
redox.ascorbate and glutathione
0.28
GLUTATHIONE PEROXIDASE 3
ID_Pisum_sativum_v2_Contig7800
AT3G63080
Q9LYB4
21.2.2
redox.ascorbate and glutathione.glutathione
-0.45
GLUTATHIONE PEROXIDASE 4
ID_Pisum_sativum_v2_Contig1327
AT4G11600
O48646
21.2.2
redox.ascorbate and glutathione.glutathione
0.19
GLUTATHIONE PEROXIDASE 6
ID270618_p.sativum_wa1_contig03738
AT2G25080
P52032
21.2
redox.ascorbate and glutathione
-0.07
GLUTATHIONE PEROXIDASE 7
ID_Pisum_sativum_v2_Contig8768
AT3G24170
P48641
21.2.2
redox.ascorbate and glutathione.glutathione
1.23
GLUTATHIONE REDUCTASE [cytoplasm]
ID_Pisum_sativum_v2_Contig4628
AT3G54660
P42770
21.2.2
redox.ascorbate and glutathione.glutathione
-0.2
GLUTATHIONE REDUCTASE [chloroplast]
ID_Pisum_sativum_v2_Contig4113
AT3G52880
Q9LFA3
21.2
redox.ascorbate and glutathione
-0.08
MONODEHYDROASCORBATE REDUCTASE 1
ID289338_p.sativum_wa1_contig19560
AT3G27820
Q9LK94
21.2.1
redox.ascorbate and glutathione.ascorbate
-0.36
MONODEHYDROASCORBATE REDUCTASE 4
ID_Pisum_sativum_v2_Contig4052
AT5G18100
Q9FK60
21.6
redox.dismutases and catalases
-0.24
SUPEROXIDE DISMUTASE 3 [Cu,Zn]
ID_Pisum_sativum_v2_Contig4187
AT3G10920
O81235
21.6
redox.dismutases and catalases
-0.04
SUPEROXIDE DISMUTASE 1 [Mn]
ID291601_p.sativum_wa1_contig26572
AT4G39640
Q8VYW6
20.2
stress.abiotic
4.74
?-GLUTAMYL TRANSPEPTIDASE
ID278435_p.sativum_wa1_contig34236
AT4G39650
Q680I5
21.2.2
redox.ascorbate and glutathione.glutathione
5.21
?-GLUTAMYL TRANSPEPTIDASE
ID_Pisum_sativum_v2_Contig4841
#N/A
#N/A
#N/A
-0.03
GLUTAMATE-CYSTEINE LIGASE
ID286494_p.sativum_wa1_contig25210
AT5G27380
P46416
21.2.2
redox.ascorbate and glutathione.glutathione
-0.29
GLUTATHIONE SYNTHETASE
ID295758_p.sativum_wa1_contig30872
AT5G27380
P46416
21.2.2
redox.ascorbate and glutathione.glutathione
-0.24
GLUTATHIONE SYNTHETASE
ID_Pisum_sativum_v2_Contig36
AT3G04120
P25858
4.1.8
glycolysis.cytosolic branch.glyceraldehyde 3-phosphate dehydrogenase (GAP-DH)
-0.57
GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE [cytoplasm]
ID_Pisum_sativum_v2_Contig6409
AT1G13440
Q9FX54
4.1.8
glycolysis.cytosolic branch.glyceraldehyde 3-phosphate dehydrogenase (GAP-DH)
0.83
GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE [cytoplasm]
ID282363_p.sativum_wa1_contig24234
AT3G05170
Q9MAA2
4.3.12
glycolysis.unclear/dually targeted.phosphoglycerate mutase
-0.76
PHOSPHOGLYCERATE MUTASE [cytoplasm]
ID290074_p.sativum_wa1_contig28839
AT3G50520
Q9SCS3
4.3.12
glycolysis.unclear/dually targeted.phosphoglycerate mutase
0.93
PHOSPHOGLYCERATE MUTASE [cytoplasm]
ID282982_p.sativum_wa1_contig26055
AT3G05170
Q9MAA2
4.3.12
glycolysis.unclear/dually targeted.phosphoglycerate mutase
1.61
PHOSPHOGLYCERATE MUTASE [cytoplasm]
ID286398_p.sativum_wa1_contig19099
AT1G74030
Q9C9C4
4.1.13
glycolysis.cytosolic branch.enolase
1.53
ENOLASE [cytoplasm]
ID_Pisum_sativum_v2_Contig4678
AT1G53310
Q9MAH0
4.1.15
glycolysis.cytosolic branch.phospho-enol-pyruvate carboxylase (PEPC)
0.51
PHOSPHO-ENOL-PYRUVATE CARBOXYLASE [cytoplasm]
ID297143_p.sativum_wa1_contig21264
AT1G53310
Q9MAH0
4.1.15
glycolysis.cytosolic branch.phospho-enol-pyruvate carboxylase (PEPC)
1.2
PHOSPHO-ENOL-PYRUVATE CARBOXYLASE [cytoplasm]
ID14082_GH719589
#N/A
#N/A
#N/A
1.7
PHOSPHO-ENOL-PYRUVATE CARBOXYLASE [cytoplasm]
ID_Pisum_sativum_v2_Contig7139
AT3G14940
Q84VW9
4.1.15
glycolysis.cytosolic branch.phospho-enol-pyruvate carboxylase (PEPC)
1.38
PHOSPHO-ENOL-PYRUVATE CARBOXYLASE [cytoplasm]
ID296901_p.sativum_wa1_contig28437
AT2G22480
Q8VYN6
4.2.4
glycolysis.plastid branch.phosphofructokinase (PFK)
1.47
PHOSPHOFRUCTOKINASE [plastid]
ID_Pisum_sativum_v2_Contig6823
AT2G22480
Q8VYN6
4.2.4
glycolysis.plastid branch.phosphofructokinase (PFK)
1.26
PHOSPHOFRUCTOKINASE [plastid]
ID265166_p.sativum_wa1_contig23674
AT1G32440
Q93Z53
4.2.14
glycolysis.plastid branch.pyruvate kinase (PK)
-0.53
PYRUVATE KINASE [plastid]
ID284266_p.sativum_wa1_contig33550
AT1G32440
Q93Z53
4.2.14
glycolysis.plastid branch.pyruvate kinase (PK)
-0.52
PYRUVATE KINASE [plastid]
ID_Pisum_sativum_v2_Contig2999
AT5G08570
Q9FNN1
4.1.14
glycolysis.cytosolic branch.pyruvate kinase (PK)
0.59
PYRUVATE KINASE [plastid]
ID277035_p.sativum_wa1_contig18485
AT5G52920
Q9FLW9
4.2.14
glycolysis.plastid branch.pyruvate kinase (PK)
-0.81
PYRUVATE KINASE [plastid]
ID272847_p.sativum_wa1_contig12510
AT3G22960
Q9LIK0
4.2.14
glycolysis.plastid branch.pyruvate kinase (PK)
-0.8
PYRUVATE KINASE [plastid]
ID_Pisum_sativum_v2_Contig4652
AT3G22960
Q9LIK0
4.2.14
glycolysis.plastid branch.pyruvate kinase (PK)
-0.5
PYRUVATE KINASE [plastid]
ID282363_p.sativum_wa1_contig24234
AT3G05170
Q9MAA2
4.3.12
glycolysis.unclear/dually targeted.phosphoglycerate mutase
-0.76
PHOSPHOGLYCERATE MUTASE [localization unclear]
ID290074_p.sativum_wa1_contig28839
AT3G50520
Q9SCS3
4.3.12
glycolysis.unclear/dually targeted.phosphoglycerate mutase
0.93
PHOSPHOGLYCERATE MUTASE [localization unclear]
ID286427_p.sativum_wa1_contig20903
AT1G22170
Q9LM13
4.2.12
glycolysis.plastid branch.phosphoglycerate mutase
2.14
PHOSPHOGLYCERATE MUTASE [localization unclear]
ID282982_p.sativum_wa1_contig26055
AT3G05170
Q9MAA2
4.3.12
glycolysis.unclear/dually targeted.phosphoglycerate mutase
1.61
PHOSPHOGLYCERATE MUTASE [localization unclear]
ID278763_p.sativum_wa1_contig17872
AT1G34430
Q9C8P0
8.1.1.2
TCA / org transformation.TCA.pyruvate DH.E2
0.97
PYRUVATE DEHYDROGENASE [E2]
ID_Pisum_sativum_v2_Contig4865
AT2G44350
P20115
8.1.2
TCA / org transformation.TCA.CS
0.73
CITRATE SYNTHASE