Species & Dataset

Experiment

Foliar Ozone Injury

  • Glycine max

  • Common name: Soybean

  • Family: Fabaceae

  • Cultivar: Glycine soja

  • Tissue: Third trifoliate

  • Ozone concentration: 75 ppb

  • Ozone exposure: 7 hours

  • Sampling time: 7 hours after ozone treatment

  • Platform: Hiseq 2000 (Illumina)

  • Year of study: 2017

  • Location: Chicago, USA

Glycine INJURY.jpeg

Title: RNA-Seq study reveals genetic responses of diverse wild soybean accessions to increased ozone levels.

 

Summary: Ozone is an air pollutant widely known to cause a decrease in productivity in many plant species, including soybean (Glycine max (L.) Merr). While the response of cultivated soybean to ozone has been studied, very little information is available regarding the ozone response of its wild relatives. Ozone-resistant wild soybean accessions were identified by measuring the response of a genetically diverse group of 66 wild soybean (Glycine soja Zucc. and Sieb.) accessions to elevated ozone levels. RNA-Seq analyses were performed on leaves of different ages from selected ozone-sensitive and ozone-resistant accessions that were subjected to treatment with an environmentally relevant level of ozone. Many more genes responded to elevated ozone in the two ozone-sensitive accessions than in the ozone-resistant accessions. Analyses of the ozone response genes indicated that leaves of different ages responded differently to ozone. Older leaves displayed a consistent reduction in expression of genes involved in photosynthesis in response to ozone, while changes in expression of defense genes dominated younger leaf tissue in response to ozone. As expected, there is a substantial difference between the response of ozone-sensitive and ozone-resistant accessions. Genes associated with photosystem 2 were substantially reduced in expression in response to ozone in the ozone-resistant accessions. A decrease in peptidase inhibitors was one of several responses specific to one of the ozone resistant accessions. The decrease in expression in genes associated with photosynthesis confirms that the photosynthetic apparatus may be an early casualty in response to moderate levels of ozone. A compromise of photosynthesis would substantially impact plant growth and seed production. However, the resistant accessions may preserve their photosynthetic apparatus in response to the ozone levels used in this study. Older leaf tissue of the ozone-resistant accessions showed a unique down-regulation of genes associated with endopeptidase inhibitor activity. This study demonstrates the existence of significant diversity in wild soybean for ozone response. Wild soybean accessions characterized in this study can be used by soybean breeders to enhance ozone tolerance of this important food crop.

 

Data repository: NCBI Gene Expression Omnibus (Project: GSE85146) (http://www.ncbi.nlm.nih.gov/geo/).

Reference: Waldeck, N., Burkey, K., Carter, T., Dickey, D., Song, Q. and Taliercio, E., 2017. RNA-Seq study reveals genetic responses of diverse wild soybean accessions to increased ozone levels. BMC genomics, 18(1), pp.1-10.

GO_acc
ontology
Gene description
407179_1st trifoliate Z-Score_row1
407179_2nd trifoliate pvalue_row1
407179_3rd trifoliate Z-Score_row2
424007_1st trifoliate pvalue_row2
424007_2nd trifoliate Z-Score_row3
424007_3rd trifoliate pvalue_row3
424123_1st trifoliate Z-Score_row4
424123_2nd trifoliate pvalue_row4
424123_3rd trifoliate Z-Score_row5
507656_1st trifoliate pvalue_row5
507656_2nd trifoliate Z-Score_row6
507656_3rd trifoliate pvalue_row6
GO:0016772
F
transferase activity, transferring phosphorus-containing groups
7.7
0
7.1
0
2.4
0.017
4.8
0.0000013
5.6
2.6e-8
7.6
0
GO:0046914
F
transition metal ion binding
-1.4
0.15
-0.14
0.89
1
0.31
2
0.049
1.4
0.17
-0.19
0.85
GO:0046915
F
transition metal ion transmembrane transporter activity
-0.89
0.37
-0.68
0.49
-3.6
0.00029
-1.1
0.28
-1.2
0.22
-1.5
0.14
GO:0000041
P
transition metal ion transport
-0.63
0.53
-0.73
0.47
-0.71
0.48
-1
0.3
-1.2
0.23
-1.3
0.19
GO:0006412
P
translation
-2
0.041
-3.4
0.0006
-2.8
0.0049
-0.72
0.47
-2.6
0.0099
-3.9
0.000095
GO:0008135
F
translation factor activity, nucleic acid binding
-1.9
0.057
-1.5
0.14
-0.33
0.74
-1.1
0.25
-1.8
0.073
-2.4
0.015
GO:0003743
F
translation initiation factor activity
-0.87
0.39
-0.46
0.64
-0.22
0.83
-0.079
0.94
-0.52
0.6
-1.3
0.21
GO:0006414
P
translational elongation
-0.69
0.49
-0.55
0.58
0.019
0.98
0.23
0.82
-0.4
0.69
-0.8
0.42
GO:0006413
P
translational initiation
-1.1
0.28
-0.48
0.63
-0.25
0.8
-0.18
0.85
-0.49
0.62
-1.3
0.2
GO:0004888
F
transmembrane receptor activity
1.6
0.11
2
0.045
1.5
0.12
2.8
0.0054
3.2
0.0013
3.6
0.00033
GO:0055085
P
transmembrane transport
2.3
0.021
1.8
0.077
1.6
0.11
-0.14
0.89
0.17
0.87
0.045
0.96
GO:0022857
F
transmembrane transporter activity
2.6
0.01
1.6
0.099
0.96
0.34
-0.55
0.58
0.57
0.57
1.4
0.17
GO:0006810
P
transport
2.2
0.027
0.45
0.66
1.6
0.11
-0.75
0.45
-0.8
0.42
0.11
0.91
GO:0030133
C
transport vesicle
2
0.045
-0.072
0.94
0.16
0.87
0.76
0.45
-0.34
0.73
-0.51
0.61
GO:0030658
C
transport vesicle membrane
2
0.045
-0.072
0.94
0.16
0.87
0.76
0.45
-0.34
0.73
-0.51
0.61
GO:0005215
F
transporter activity
0.27
0.78
-0.29
0.77
0.24
0.81
-2.1
0.037
-0.89
0.37
0.3
0.77
GO:0005992
P
trehalose biosynthetic process
1.5
0.12
1.9
0.058
1
0.31
-1.3
0.2
0.35
0.73
0.15
0.88
GO:0005991
P
trehalose metabolic process
1.5
0.13
1.8
0.066
1
0.31
-1.1
0.27
0.43
0.67
0.13
0.9
GO:0004806
F
triglyceride lipase activity
3
0.0026
2.9
0.0038
1.1
0.26
1.4
0.16
2.7
0.0075
2.1
0.032
GO:0043039
P
tRNA aminoacylation
-0.69
0.49
-1.6
0.11
-0.76
0.45
-1.4
0.17
-1.5
0.14
-2
0.044
GO:0006418
P
tRNA aminoacylation for protein translation
-0.64
0.52
-1.6
0.11
-0.78
0.44
-1.4
0.17
-1.5
0.14
-2
0.043
GO:0006399
P
tRNA metabolic process
-1.7
0.096
-3
0.0026
-1.2
0.21
-2.2
0.027
-2.7
0.0066
-2.9
0.0041
GO:0008175
F
tRNA methyltransferase activity
0.0079
0.99
-1.1
0.28
-0.95
0.34
-1
0.31
-1.4
0.15
0.06
0.95
GO:0006400
P
tRNA modification
0.056
0.96
-1.3
0.18
-1
0.31
-0.73
0.47
-1
0.31
0.85
0.4
GO:0008033
P
tRNA processing
-1.7
0.085
-2.7
0.0075
-0.98
0.33
-1.8
0.067
-2.4
0.017
-2
0.043
GO:0015631
F
tubulin binding
-0.48
0.63
-0.5
0.62
-0.81
0.42
-0.72
0.47
-2
0.045
-0.3
0.76
GO:0000156
F
two-component response regulator activity
0.3
0.77
-2.2
0.031
-0.43
0.67
-1.8
0.077
-1.5
0.13
-0.41
0.68
GO:0000155
F
two-component sensor activity
0.68
0.49
-0.42
0.68
0.37
0.71
-2
0.042
-1.2
0.21
0.043
0.97
GO:0000160
P
two-component signal transduction system (phosphorelay)
-0.27
0.79
-2.5
0.013
-0.78
0.43
-2.7
0.0065
-2.1
0.034
-0.51
0.61
GO:0000151
C
ubiquitin ligase complex
4.5
0.0000062
6.2
5e-10
2.2
0.026
5
5.3e-7
4.4
0.0000094
4.2
0.000024
GO:0004221
F
ubiquitin thiolesterase activity
1.7
0.084
0.6
0.55
0.47
0.64
0.38
0.71
-0.12
0.91
0.59
0.56
GO:0006511
P
ubiquitin-dependent protein catabolic process
3.2
0.0012
1.7
0.084
0.7
0.49
0.66
0.51
-0.32
0.75
0.73
0.46
GO:0004842
F
ubiquitin-protein ligase activity
4.7
0.0000029
6.2
5e-10
2.3
0.024
5.1
3.3e-7
4.5
0.000007
4.3
0.000016
GO:0035251
F
UDP-glucosyltransferase activity
-0.038
0.97
-0.37
0.71
-0.9
0.37
-1.2
0.21
-1
0.3
-1.8
0.07
GO:0008194
F
UDP-glycosyltransferase activity
1.4
0.15
1.1
0.27
0.12
0.91
0.46
0.65
0.43
0.67
-0.82
0.41
GO:0008762
F
UDP-N-acetylmuramate dehydrogenase activity
0.39
0.7
0.25
0.8
-0.33
0.74
0.46
0.64
1.8
0.065
1.6
0.11
GO:0051082
F
unfolded protein binding
2
0.042
2.7
0.0072
2.1
0.033
2.5
0.013
3.3
0.00092
5.3
1.1e-7
GO:0031982
C
vesicle
2.6
0.01
-0.088
0.93
0.2
0.84
0.83
0.41
-0.26
0.79
-0.53
0.6
GO:0030120
C
vesicle coat
2.6
0.01
-0.088
0.93
0.2
0.84
0.83
0.41
-0.26
0.79
-0.53
0.6
GO:0012506
C
vesicle membrane
2.6
0.01
-0.088
0.93
0.2
0.84
0.83
0.41
-0.26
0.79
-0.53
0.6
GO:0016192
P
vesicle-mediated transport
3.2
0.0012
0.41
0.68
-0.21
0.84
1.2
0.25
-0.01
0.99
0.059
0.95
GO:0070279
F
vitamin B6 binding
3.6
0.00036
1.5
0.13
-0.38
0.71
2.3
0.023
1.4
0.17
-0.055
0.96
GO:0019842
F
vitamin binding
3.4
0.00057
1.5
0.15
-0.63
0.53
2.2
0.03
1.6
0.11
0.06
0.95
GO:0009110
P
vitamin biosynthetic process
0.8
0.42
0.52
0.6
-0.23
0.82
-0.61
0.54
0.12
0.91
0.73
0.46
GO:0006766
P
vitamin metabolic process
0.8
0.42
0.52
0.6
-0.23
0.82
-0.61
0.54
0.12
0.91
0.73
0.46
GO:0042364
P
water-soluble vitamin biosynthetic process
0.8
0.42
0.52
0.6
-0.23
0.82
-0.61
0.54
0.12
0.91
0.73
0.46
GO:0006767
P
water-soluble vitamin metabolic process
0.8
0.42
0.52
0.6
-0.23
0.82
-0.61
0.54
0.12
0.91
0.73
0.46
GO:0016762
F
xyloglucan:xyloglucosyl transferase activity
-4.6
0.0000046
-5.7
9.2e-9
-1.4
0.16
-5.6
2.2e-8
-5.6
1.8e-8
-7.6
0
GO:0008270
F
zinc ion binding
-0.29
0.77
0.87
0.38
1.3
0.2
0.6
0.55
-0.23
0.82
0.065
0.95
GO:0044282
P
small molecule catabolic process
0.93
0.35
0.13
0.9
0.41
0.68
1.7
0.096
1.2
0.22
-0.55
0.59
GO:0044281
P
small molecule metabolic process
0.98
0.33
-0.66
0.51
-0.72
0.47
0.74
0.46
0.13
0.89
-1.1
0.29
GO:0006814
P
sodium ion transport
0.018
0.99
0.062
0.95
-4.3
0.000014
-0.21
0.83
0.53
0.6
-1.6
0.11
GO:0015298
F
solute:cation antiporter activity
1
0.29
0.46
0.65
0.94
0.35
-0.2
0.84
-0.28
0.78
1.2
0.22
GO:0015294
F
solute:cation symporter activity
1.5
0.15
0.45
0.65
-0.15
0.88
-0.19
0.85
0.55
0.58
-0.3
0.76
GO:0015299
F
solute:hydrogen antiporter activity
1
0.29
0.46
0.65
0.94
0.35
-0.2
0.84
-0.28
0.78
1.2
0.22
GO:0015300
F
solute:solute antiporter activity
0.47
0.64
-0.19
0.85
0.9
0.37
-0.83
0.41
-0.86
0.39
0.57
0.57
GO:0006694
P
steroid biosynthetic process
0.24
0.81
0.48
0.63
2.4
0.016
1.6
0.11
1.4
0.15
1.5
0.14
GO:0016229
F
steroid dehydrogenase activity
0.38
0.71
0.59
0.56
2.4
0.014
1.7
0.09
1.5
0.14
1.5
0.14
GO:0033764
F
steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
0.38
0.71
0.59
0.56
2.4
0.014
1.7
0.09
1.5
0.14
1.5
0.14
GO:0008202
P
steroid metabolic process
0.25
0.81
0.48
0.63
2.2
0.026
1.6
0.11
1.4
0.16
1.5
0.14
GO:0003735
F
structural constituent of ribosome
-1.2
0.23
-2.7
0.0074
-2.9
0.0042
0.37
0.71
-1.6
0.12
-2.6
0.0093
GO:0005198
F
structural molecule activity
-1.9
0.057
-3.6
0.00037
-1.8
0.067
-0.54
0.59
-2.9
0.0043
-3.8
0.00015
GO:0043566
F
structure-specific DNA binding
-0.02
0.98
-0.56
0.58
-0.42
0.67
-0.16
0.87
-0.66
0.51
0.018
0.99
GO:0022838
F
substrate-specific channel activity
1.8
0.068
2
0.044
1.2
0.24
0.89
0.37
1.6
0.11
2.7
0.0061
GO:0022891
F
substrate-specific transmembrane transporter activity
0.82
0.41
0.39
0.7
-0.49
0.62
-1.4
0.15
-0.7
0.48
0.85
0.39
GO:0022892
F
substrate-specific transporter activity
1
0.32
0.5
0.62
-0.52
0.6
-1.5
0.14
-0.67
0.5
0.92
0.36
GO:0071212
C
subsynaptic reticulum
2
0.041
1.7
0.095
1.2
0.23
2.5
0.013
1.5
0.13
1.7
0.094
GO:0015116
F
sulfate transmembrane transporter activity
-0.58
0.56
-0.44
0.66
-0.77
0.44
-3.2
0.0014
-0.87
0.39
-0.61
0.54
GO:0008272
P
sulfate transport
-0.58
0.56
-0.44
0.66
-0.77
0.44
-3.2
0.0014
-0.87
0.39
-0.61
0.54
GO:0000096
P
sulfur amino acid metabolic process
1.1
0.29
0.63
0.53
0.41
0.68
2.1
0.036
1.5
0.13
-0.48
0.63
GO:0044272
P
sulfur compound biosynthetic process
-0.18
0.86
0.23
0.82
0.14
0.89
0.52
0.6
0.51
0.61
-0.7
0.48
GO:0006790
P
sulfur metabolic process
-0.23
0.82
-0.34
0.73
0.2
0.85
0.19
0.85
-0.18
0.86
-1.4
0.17
GO:0015293
F
symporter activity
1.5
0.15
0.45
0.65
-0.15
0.88
-0.19
0.85
0.55
0.58
-0.3
0.76
GO:0016114
P
terpenoid biosynthetic process
-0.32
0.75
-1.9
0.063
0.78
0.44
-1.7
0.085
-1.3
0.2
-0.85
0.4
GO:0006721
P
terpenoid metabolic process
-0.32
0.75
-1.9
0.063
0.78
0.44
-1.7
0.085
-1.3
0.2
-0.85
0.4
GO:0046906
F
tetrapyrrole binding
-1
0.3
0.35
0.72
0.76
0.45
2.2
0.025
3
0.0024
1.1
0.28
GO:0033014
P
tetrapyrrole biosynthetic process
-0.025
0.98
-1.8
0.077
-0.63
0.53
-1.2
0.24
-1.8
0.075
-1.9
0.06
GO:0033015
P
tetrapyrrole catabolic process
1.5
0.14
1.9
0.052
1.8
0.08
0.9
0.37
0.12
0.91
0.83
0.4
GO:0033013
P
tetrapyrrole metabolic process
0.72
0.47
-0.54
0.59
0.34
0.73
-0.55
0.58
-1.5
0.14
-1.2
0.23
GO:0016790
F
thiolester hydrolase activity
1.9
0.058
-0.22
0.83
0.19
0.85
0.26
0.8
-0.23
0.82
0.18
0.86
GO:0004298
F
threonine-type endopeptidase activity
3.6
0.00034
1.8
0.071
0.96
0.34
1.7
0.085
0.44
0.66
1.1
0.29
GO:0070003
F
threonine-type peptidase activity
3.6
0.00034
1.8
0.071
0.96
0.34
1.7
0.085
0.44
0.66
1.1
0.29
GO:0009579
C
thylakoid
-9.8
0
-6
2.4e-9
-4.8
0.0000017
-7.6
0
-6.6
0
-5.1
3.2e-7
GO:0044436
C
thylakoid part
-8.1
0
-5.6
2.7e-8
-2.5
0.011
-5.8
6.3e-9
-5.6
2.3e-8
-4.9
9.8e-7
GO:0006350
P
transcription
2.6
0.0081
4.2
0.000022
1.9
0.054
1.7
0.085
4.4
0.0000091
3
0.0025
GO:0003700
F
transcription factor activity
3.5
0.00051
6.8
0
2.4
0.019
6
1.8e-9
7.3
0
4.1
0.000037
GO:0008134
F
transcription factor binding
0.79
0.43
0.61
0.54
-2.9
0.004
-1.8
0.078
-1.2
0.21
-1.2
0.24
GO:0006352
P
transcription initiation
-1.1
0.25
-1.2
0.23
-0.44
0.66
-2.1
0.037
-2.7
0.0067
-0.84
0.4
GO:0016986
F
transcription initiation factor activity
-1.4
0.15
-1.3
0.19
0.19
0.85
-2.5
0.014
-2.9
0.0033
-1.3
0.19
GO:0030528
F
transcription regulator activity
3.3
0.00082
5.8
6.7e-9
1.4
0.16
5
5e-7
6.3
2.8e-10
3.6
0.00035
GO:0006351
P
transcription, DNA-dependent
2.6
0.0081
4.3
0.000018
1.9
0.06
1.7
0.083
4.5
0.0000075
3
0.0025
GO:0016740
F
transferase activity
6.8
0
5.8
7e-9
1.3
0.19
5.6
2.6e-8
5.1
2.9e-7
5
6.5e-7
GO:0016746
F
transferase activity, transferring acyl groups
2
0.042
2.6
0.0087
-0.8
0.42
2.5
0.012
2.5
0.012
1
0.29
GO:0016747
F
transferase activity, transferring acyl groups other than amino-acyl groups
2.3
0.023
2.7
0.007
-0.78
0.43
2.9
0.0038
3
0.0026
1.5
0.14
GO:0046912
F
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
0.8
0.43
1.4
0.17
0.47
0.64
0.22
0.83
0.54
0.59
-0.19
0.85
GO:0016765
F
transferase activity, transferring alkyl or aryl (other than methyl) groups
-0.17
0.87
0.19
0.85
1.1
0.26
2.4
0.018
2.1
0.034
-2
0.045
GO:0016757
F
transferase activity, transferring glycosyl groups
0.7
0.48
-0.69
0.49
-2.3
0.023
1.3
0.21
-0.54
0.59
-3.4
0.00064
GO:0016758
F
transferase activity, transferring hexosyl groups
0.73
0.47
-0.21
0.83
-1.5
0.13
1.5
0.13
0.32
0.75
-2.8
0.0054
GO:0016741
F
transferase activity, transferring one-carbon groups
0.03
0.98
-0.6
0.55
1.7
0.083
1.6
0.12
0.52
0.6
1.9
0.058
GO:0004540
F
ribonuclease activity
1.9
0.064
1.4
0.18
2.8
0.0059
0.029
0.98
0.32
0.75
1.3
0.2
GO:0004525
F
ribonuclease III activity
1.2
0.22
1.4
0.16
2
0.049
-0.25
0.81
-0.37
0.71
0.015
0.99
GO:0030529
C
ribonucleoprotein complex
-0.93
0.35
-2.6
0.009
-2.8
0.0047
0.41
0.68
-1.6
0.11
-2.5
0.013
GO:0022613
P
ribonucleoprotein complex biogenesis
0.83
0.41
-0.86
0.39
-0.77
0.44
-0.92
0.36
-1
0.32
-0.21
0.83
GO:0009119
P
ribonucleoside metabolic process
-0.063
0.95
-0.19
0.85
-0.024
0.98
-0.24
0.81
-0.27
0.79
0.023
0.98
GO:0009156
P
ribonucleoside monophosphate biosynthetic process
1.1
0.25
-0.11
0.91
0.16
0.87
-0.58
0.56
-0.93
0.35
-0.83
0.41
GO:0009161
P
ribonucleoside monophosphate metabolic process
1.1
0.25
-0.11
0.91
0.16
0.87
-0.58
0.56
-0.93
0.35
-0.83
0.41
GO:0009201
P
ribonucleoside triphosphate biosynthetic process
-2
0.043
-0.57
0.57
-1.7
0.096
-0.52
0.6
-0.95
0.34
-0.56
0.57
GO:0009203
P
ribonucleoside triphosphate catabolic process
-2.9
0.0035
-3.1
0.002
-0.48
0.63
-1.5
0.12
-2.1
0.04
-2.1
0.035
GO:0009199
P
ribonucleoside triphosphate metabolic process
-3.5
0.00049
-2.6
0.01
-1.5
0.13
-1.4
0.15
-2.1
0.035
-1.9
0.061
GO:0032553
F
ribonucleotide binding
9.5
0
7.1
0
3.6
0.00035
3.9
0.000085
4.6
0.0000034
7.5
0
GO:0009260
P
ribonucleotide biosynthetic process
-0.99
0.32
-0.52
0.6
-1.3
0.21
-0.76
0.45
-1.3
0.19
-0.94
0.35
GO:0009261
P
ribonucleotide catabolic process
-2.9
0.0035
-3.1
0.002
-0.48
0.63
-1.5
0.12
-2.1
0.04
-2.1
0.035
GO:0009259
P
ribonucleotide metabolic process
-2.6
0.0096
-2.3
0.02
-1.3
0.2
-1.6
0.12
-2.3
0.021
-2
0.041
GO:0005840
C
ribosome
-1.2
0.23
-2.7
0.0074
-2.9
0.0042
0.37
0.71
-1.6
0.12
-2.6
0.0093
GO:0042254
P
ribosome biogenesis
0.72
0.47
-0.87
0.38
-0.79
0.43
-0.92
0.36
-1
0.32
-0.2
0.84
GO:0003723
F
RNA binding
-0.13
0.89
-1.5
0.12
0.59
0.56
-2.3
0.022
-2.9
0.0037
-2.5
0.011
GO:0032774
P
RNA biosynthetic process
2.6
0.0081
4.3
0.000018
1.9
0.06
1.8
0.072
4.5
0.0000078
3
0.0025
GO:0016070
P
RNA metabolic process
2.2
0.03
2.9
0.0033
1.3
0.19
0.49
0.62
2.7
0.0068
1.4
0.16
GO:0008173
F
RNA methyltransferase activity
-0.43
0.66
-1.3
0.2
-0.91
0.36
-1.4
0.17
-1.5
0.13
-0.92
0.36
GO:0009451
P
RNA modification
-0.24
0.81
-1.1
0.27
-1.1
0.27
-1.1
0.25
-1.3
0.21
-1
0.3
GO:0034062
F
RNA polymerase activity
1.2
0.24
0.33
0.74
0.13
0.9
-0.087
0.93
-0.37
0.71
0.74
0.46
GO:0006396
P
RNA processing
-0.29
0.77
-1.8
0.067
-0.34
0.74
-2.5
0.012
-3
0.0029
-2.3
0.019
GO:0019843
F
rRNA binding
-0.94
0.35
-1.3
0.2
-1.5
0.13
-1.2
0.23
-0.61
0.54
-1.2
0.24
GO:0016072
P
rRNA metabolic process
-0.19
0.85
-1.2
0.22
-0.51
0.61
-1.5
0.14
-1.4
0.17
-0.32
0.75
GO:0006364
P
rRNA processing
-0.19
0.85
-1.2
0.22
-0.51
0.61
-1.5
0.14
-1.4
0.17
-0.32
0.75
GO:0008757
F
S-adenosylmethionine-dependent methyltransferase activity
-0.74
0.46
-1.6
0.1
-1.3
0.2
-1.3
0.18
-1.2
0.22
-1
0.31
GO:0015291
F
secondary active transmembrane transporter activity
0.97
0.33
-0.052
0.96
0.24
0.81
-1.7
0.091
-0.38
0.7
-1.1
0.27
GO:0019748
P
secondary metabolic process
0.52
0.6
-0.54
0.59
0.4
0.69
-0.68
0.5
-0.34
0.74
-0.66
0.51
GO:0046903
P
secretion
1.5
0.12
0.88
0.38
-0.62
0.54
0.9
0.37
-0.25
0.8
0.36
0.72
GO:0032940
P
secretion by cell
1.5
0.12
0.88
0.38
-0.62
0.54
0.9
0.37
-0.25
0.8
0.36
0.72
GO:0043565
F
sequence-specific DNA binding
2.9
0.0036
5.5
4.8e-8
3.4
0.00067
5.6
2.3e-8
5.2
1.7e-7
4.9
9.3e-7
GO:0009071
P
serine family amino acid catabolic process
-2.4
0.018
-1.5
0.14
-0.11
0.92
-1.4
0.18
-1.1
0.29
-1.1
0.27
GO:0009069
P
serine family amino acid metabolic process
-1.4
0.16
-1.2
0.23
0.46
0.65
-0.29
0.77
-0.31
0.76
-0.42
0.67
GO:0017171
F
serine hydrolase activity
-2.9
0.0035
-2.5
0.011
-2.8
0.0047
-3.7
0.00019
-2.6
0.0087
-2.9
0.0037
GO:0004185
F
serine-type carboxypeptidase activity
-0.49
0.63
-0.23
0.82
-0.89
0.38
-1.9
0.06
-0.39
0.7
-0.65
0.52
GO:0004252
F
serine-type endopeptidase activity
-2.6
0.0091
-2.7
0.006
-2.7
0.0078
-2.8
0.0048
-2.3
0.02
-2.7
0.0076
GO:0070008
F
serine-type exopeptidase activity
-0.21
0.84
-0.23
0.82
-0.8
0.43
-1.8
0.074
-0.3
0.76
-0.52
0.6
GO:0008236
F
serine-type peptidase activity
-2.9
0.0035
-2.5
0.011
-2.8
0.0047
-3.7
0.00019
-2.6
0.0087
-2.9
0.0037
GO:0016987
F
sigma factor activity
-1.4
0.15
-1.3
0.19
0.19
0.85
-2.5
0.014
-2.9
0.0033
-1.3
0.19
GO:0004871
F
signal transducer activity
0.31
0.76
-1.3
0.18
-0.25
0.8
-1.8
0.073
-0.89
0.37
1
0.31
GO:0007165
P
signal transduction
2.6
0.0084
1.4
0.17
0.68
0.5
0.63
0.53
0.56
0.57
1.3
0.19
GO:0023060
P
signal transmission
2.2
0.03
0.35
0.72
0.23
0.82
-0.2
0.84
-0.19
0.85
0.98
0.33
GO:0023052
P
signaling
5.1
4e-7
3.8
0.00015
2
0.047
3.2
0.0012
3.3
0.00082
4.7
0.0000026
GO:0023033
P
signaling pathway
2.9
0.0037
1.8
0.074
1
0.3
1.5
0.14
1.1
0.28
1.6
0.12
GO:0023046
P
signaling process
2.2
0.03
0.35
0.72
0.23
0.82
-0.2
0.84
-0.19
0.85
0.98
0.33
GO:0019787
F
small conjugating protein ligase activity
4.7
0.0000029
6.2
5e-10
2.3
0.024
5.1
3.3e-7
4.5
0.000007
4.3
0.000016
GO:0007264
P
small GTPase mediated signal transduction
2
0.041
1.6
0.12
1.5
0.14
1.9
0.063
0.63
0.53
0.37
0.71
GO:0005083
F
small GTPase regulator activity
0.2
0.84
-1.6
0.12
-0.37
0.71
-1.1
0.26
-1.4
0.16
-0.64
0.52
GO:0044283
P
small molecule biosynthetic process
2.8
0.0048
0.92
0.36
-0.28
0.78
2.8
0.0059
2.5
0.011
0.051
0.96
GO:0005088
F
Ras guanyl-nucleotide exchange factor activity
-2.2
0.025
-2
0.041
-0.89
0.37
-3
0.0026
-3.2
0.0012
-1.6
0.12
GO:0007265
P
Ras protein signal transduction
2.5
0.013
0.79
0.43
0.71
0.48
0.51
0.61
0.33
0.74
0.24
0.81
GO:0004872
F
receptor activity
1.6
0.11
2
0.045
1.5
0.12
2.8
0.0054
3.2
0.0013
3.6
0.00033
GO:0048544
P
recognition of pollen
5.2
2e-7
6.5
0
3.3
0.00082
6.9
0
7.2
0
7.4
0
GO:0050789
P
regulation of biological process
2.4
0.016
4.5
0.0000059
2
0.042
1.9
0.055
3.9
0.000094
2.7
0.007
GO:0065008
P
regulation of biological quality
-2.8
0.0047
-0.87
0.38
-0.72
0.47
-2
0.05
-1.5
0.12
-1.4
0.18
GO:0009889
P
regulation of biosynthetic process
2.5
0.012
4.5
0.0000072
2.3
0.024
2
0.048
4.8
0.0000017
3
0.0023
GO:0050790
P
regulation of catalytic activity
-0.28
0.78
-0.8
0.42
-1.8
0.066
-1.2
0.23
-0.84
0.4
-1.8
0.069
GO:0010646
P
regulation of cell communication
1.9
0.054
0.7
0.48
0.68
0.49
0.43
0.66
0.22
0.82
0.16
0.87
GO:0031326
P
regulation of cellular biosynthetic process
2.5
0.012
4.5
0.0000072
2.3
0.024
2
0.048
4.8
0.0000017
3
0.0023
GO:0031323
P
regulation of cellular metabolic process
2.5
0.012
4.5
0.0000055
2.3
0.024
2
0.046
4.7
0.0000021
3
0.0027
GO:0050794
P
regulation of cellular process
2.3
0.019
4.5
0.0000059
2
0.043
1.9
0.059
4
0.000073
2.6
0.0083
GO:0010468
P
regulation of gene expression
2.6
0.0087
4.5
0.000007
2.3
0.024
1.9
0.053
4.7
0.0000032
3.1
0.0018
GO:0043087
P
regulation of GTPase activity
2.4
0.018
0.86
0.39
0.73
0.46
0.38
0.7
0.41
0.68
0.31
0.76
GO:0051336
P
regulation of hydrolase activity
1.8
0.071
0.79
0.43
0.71
0.48
0.33
0.74
0.33
0.74
0.24
0.81
GO:0043549
P
regulation of kinase activity
2.3
0.024
1.4
0.17
1
0.29
0.96
0.34
0.82
0.41
0.23
0.82
GO:0010556
P
regulation of macromolecule biosynthetic process
2.5
0.012
4.5
0.0000072
2.3
0.024
2
0.048
4.8
0.0000017
3
0.0023
GO:0060255
P
regulation of macromolecule metabolic process
2.6
0.0086
4.5
0.0000073
2.3
0.024
2
0.045
4.7
0.0000025
3.1
0.0018
GO:0019222
P
regulation of metabolic process
2.6
0.0086
4.5
0.0000056
2.3
0.023
2
0.042
4.7
0.0000031
3.1
0.0021
GO:0065009
P
regulation of molecular function
-0.39
0.7
-0.91
0.36
-1.8
0.069
-1.2
0.23
-0.93
0.35
-1.8
0.069
GO:0051171
P
regulation of nitrogen compound metabolic process
2.6
0.01
4.5
0.0000069
2.2
0.028
2
0.046
4.8
0.0000016
3.1
0.0022
GO:0019219
P
regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
2.6
0.01
4.5
0.0000069
2.2
0.028
2
0.046
4.8
0.0000016
3.1
0.0022
GO:0019220
P
regulation of phosphate metabolic process
1.6
0.1
1.3
0.21
0.99
0.32
0.86
0.39
0.7
0.49
0.16
0.87
GO:0051174
P
regulation of phosphorus metabolic process
1.6
0.1
1.3
0.21
0.99
0.32
0.86
0.39
0.7
0.49
0.16
0.87
GO:0042325
P
regulation of phosphorylation
2.3
0.024
1.4
0.17
1
0.29
0.96
0.34
0.82
0.41
0.23
0.82
GO:0080090
P
regulation of primary metabolic process
2.5
0.012
4.5
0.0000073
2.3
0.024
2
0.047
4.8
0.0000018
3
0.0024
GO:0045859
P
regulation of protein kinase activity
2.3
0.024
1.4
0.17
1
0.29
0.96
0.34
0.82
0.41
0.23
0.82
GO:0032318
P
regulation of Ras GTPase activity
2.4
0.018
0.86
0.39
0.73
0.46
0.38
0.7
0.41
0.68
0.31
0.76
GO:0046578
P
regulation of Ras protein signal transduction
2.5
0.013
0.79
0.43
0.71
0.48
0.51
0.61
0.33
0.74
0.24
0.81
GO:0051252
P
regulation of RNA metabolic process
2.6
0.0092
4.6
0.0000054
2.2
0.028
1.9
0.056
4.8
0.0000016
3.1
0.0021
GO:0009966
P
regulation of signal transduction
1.9
0.054
0.7
0.48
0.68
0.49
0.43
0.66
0.22
0.82
0.16
0.87
GO:0023051
P
regulation of signaling process
1.9
0.054
0.7
0.48
0.68
0.49
0.43
0.66
0.22
0.82
0.16
0.87
GO:0051056
P
regulation of small GTPase mediated signal transduction
2.5
0.013
0.79
0.43
0.71
0.48
0.51
0.61
0.33
0.74
0.24
0.81
GO:0045449
P
regulation of transcription
2.6
0.01
4.5
0.0000065
2.2
0.028
1.9
0.057
4.8
0.0000019
3.1
0.0021
GO:0006355
P
regulation of transcription, DNA-dependent
2.6
0.0092
4.6
0.0000054
2.2
0.028
1.9
0.056
4.8
0.0000016
3.1
0.0021
GO:0051338
P
regulation of transferase activity
2.3
0.024
1.4
0.17
1
0.29
0.96
0.34
0.82
0.41
0.23
0.82
GO:0000003
P
reproduction
5.3
1.2e-7
6.5
0
3.5
0.00056
6.8
0
7.2
0
7.4
0
GO:0022414
P
reproductive process
5.3
1.2e-7
6.5
0
3.5
0.00056
6.8
0
7.2
0
7.4
0
GO:0009607
P
response to biotic stimulus
0.88
0.38
0.65
0.51
5.6
2.5e-8
4.2
0.000029
4.4
0.0000087
5.1
3.7e-7
GO:0042221
P
response to chemical stimulus
-0.000085
1
0.93
0.35
1
0.31
-0.67
0.51
1.9
0.052
-1.1
0.28
GO:0006974
P
response to DNA damage stimulus
0.7
0.48
0.24
0.81
-0.23
0.82
-0.36
0.72
-1.2
0.23
0.85
0.4
GO:0042493
P
response to drug
0.3
0.76
0.042
0.97
-0.044
0.97
-1.5
0.14
0.032
0.97
-1.6
0.11
GO:0009719
P
response to endogenous stimulus
-2.1
0.032
-1.9
0.059
-0.7
0.48
-3.6
0.00037
-2.2
0.028
-1.4
0.16
GO:0009605
P
response to external stimulus
0.77
0.44
-0.74
0.46
-0.033
0.97
-0.13
0.9
0.48
0.63
0.76
0.45
GO:0009725
P
response to hormone stimulus
-2.1
0.032
-1.9
0.059
-0.7
0.48
-3.6
0.00037
-2.2
0.028
-1.4
0.16
GO:0010033
P
response to organic substance
-2
0.047
-1.8
0.07
-0.64
0.52
-3.3
0.0011
-2.2
0.031
-1.5
0.13
GO:0006979
P
response to oxidative stress
1.2
0.24
2.7
0.0063
2.1
0.035
3
0.003
4.7
0.0000023
0.93
0.35
GO:0050896
P
response to stimulus
1.1
0.26
1.2
0.24
3.5
0.0004
1.7
0.091
2.6
0.0082
2.4
0.018
GO:0006950
P
response to stress
2.1
0.034
2.3
0.02
4.7
0.0000023
4.5
0.0000085
4.4
0.000013
4.3
0.000016
GO:0005089
F
Rho guanyl-nucleotide exchange factor activity
-2.2
0.025
-2
0.041
-0.89
0.37
-3
0.0026
-3.2
0.0012
-1.6
0.12
GO:0015035
F
protein disulfide oxidoreductase activity
-1.6
0.1
0.84
0.4
0.35
0.72
0.14
0.89
0.44
0.66
-0.18
0.86