Species & Dataset
Experiment
Foliar ozone injury
  • Arabidopsis thaliana

  • Common name: Thale cress, Mouse-ear cress

  • Family: Brassicaceae

  • Cultivar: Col-0 (wild type), ein2 (Ethylene signaling deficient), sid2 (Salicylic acid biosynthesis deficient)

  • Tissue: Rosettes

  • Ozone concentration: 200 nL L-1

  • Ozone exposure: 24 hours

  • Platform: Macroarray

  • Year of study: 2009

  • Location: Japan

Arabidopsis_injury.png

Title:  Ethylene and salicylic acid control glutathione biosynthesis in ozone-exposed Arabidopsis thaliana.

Summary: Ozone produces reactive oxygen species and induces the synthesis of phytohormones, including ethylene and salicylic acid. These phytohormones act as signal molecules that enhance cell death in response to ozone exposure. However, some studies have shown that ethylene and salicylic acid can instead decrease the magnitude of ozone-induced cell death. Therefore, we studied the defensive roles of ethylene and salicylic acid against ozone.Unlike the wild-type, Col-0, Arabidopsis mutants deficient in ethylene signaling (ein2) or salicylic acid biosynthesis (sid2) generated high levels of superoxide and exhibited visible leaf injury, indicating that ethylene and salicylic acid can reduce ozone damage. Macroarray analysis suggested that the ethylene and salicylic acid defects influenced glutathione (GSH) metabolism. Increases in the reduced form of GSH occurred in Col-0 6 h after ozone exposure, but little GSH was detected in ein2 and sid2 mutants, suggesting that GSH levels were affected by ethylene or salicylic acid signaling. We performed gene expression analysis by real-time polymerase chain reaction using genes involved in GSH metabolism. Induction of γ-glutamylcysteine synthetase (GSH1), glutathione synthetase (GSH2), and glutathione reductase 1 (GR1) expression occurred normally in Col-0, but at much lower levels in ein2 and sid2. Enzymatic activities of GSH1 and GSH2 in ein2 and sid2 were significantly lower than in Col-0. Moreover, ozone-induced leaf damage observed in ein2 and sid2 was mitigated by artificial elevation of GSH content. Our results suggest that ethylene and salicylic acid protect against ozone-induced leaf injury by increasing de novo biosynthesis of GSH.

Reference: Yoshida, S., Tamaoki, M., Ioki, M., Ogawa, D., Sato, Y., Aono, M., Kubo, A., Saji, S., Saji, H., Satoh, S. and Nakajima, N., 2009. Ethylene and salicylic acid control glutathione biosynthesis in ozone‐exposed Arabidopsis thaliana. Physiologia Plantarum, 136(3), pp.284-298.

sid2

ein2

Col-0

Gene
Gene annotation
MIPS Code
0 h
3 h
6 h
12 h
0 h
3 h
6 h
12 h
0 h
3 h
6 h
12 h
SEC61β
Protein transport protein
AT2G45070
0.82
1.41
0.98
0.84
0.42
0.88
0.99
0.75
0.48
1
0.58
0.81
PDIL
Protein disulfide isomerase like
AT2g47470
0.52
1.07
1.01
0.73
0.26
0.61
1
0.73
0.39
0.6
0.55
0.56
PDIL2
Protein disulfide isomerase like 2
AT3G54960
0.17
0.24
0.38
0.2
0.15
0.38
0.38
0.36
0.18
0.24
0.27
0.19
CRT3
Calreticulin 3
AT1G08450
0.23
0.6
0.63
0.42
0.19
0.51
0.59
0.7
0.2
0.37
0.45
0.38
CRT2
Calreticulin 2
AT1G09210
0.51
0.91
1.04
0.65
0.33
0.73
0.93
0.86
0.38
0.78
1.07
0.77
CRT1
Calreticulin 1
AT1G56340
0.6
1.16
1.06
0.74
0.31
0.92
1.02
0.95
0.37
1.06
1.09
0.8
CNX1
Calnexin 1
AT5G61790
0.52
1.12
1.02
0.7
0.23
1.02
0.96
0.94
0.32
0.92
0.86
0.68
CNX2
Calnexin 2
AT5G07340
0.31
0.73
0.66
0.37
0.14
0.47
0.5
0.49
0.26
0.61
0.52
0.45
RBP
Ribophorin I family protein
AT2G01720
0.39
0.85
0.64
0.5
0.14
0.51
0.52
0.47
0.33
0.58
0.48
0.34
TTR
Tetratricopeptide repeat
AT4G22670
0.49
1.01
0.77
0.61
0.25
0.5
0.63
0.53
0.39
0.77
0.57
0.5
CCP
Peptidyl-prolyl cis-trans isomerase
AT2G47320
0.51
1.12
0.86
0.63
0.23
0.5
0.71
0.56
0.46
0.83
0.66
0.5
TTP
Tmp21 precursor
AT1G14010
0.25
0.68
0.64
0.38
0.11
0.41
0.57
0.4
0.24
0.46
0.42
0.31
Control genes
EF-1α
Elongation factor 1-alpha
AT1G07940
1
1
1
1
1
1
1
1
1
1
1
1
18SrRNA
18S rRNA
AT2G01010
0.72
0.87
0.76
0.47
0.77
0.79
0.64
0.53
0.6
0.78
0.55
0.5
TUBULIN4
Tubulin beta-4 chain
AT5G44340
0.69
1.19
0.83
0.58
0.57
0.74
0.92
0.69
0.54
0.62
0.65
0.52
ACTIN8
Actin 8
AT1G49240
0.75
1.21
0.67
0.48
0.53
0.75
0.57
0.58
0.52
0.8
0.64
0.49
LOX1
Lipoxygenase 1
AT1G55020
0.22
0.39
0.35
0.23
0.16
0.32
0.27
0.4
0.11
0.14
0.27
0.23
LOX2
Lipoxygenase 2
AT3G45140
0.64
1.37
2.34
1.49
0.53
1.53
1.79
1.5
0.28
1.23
1.73
1.27
AOS
Allene oxide synthase
AT5G42650
0.65
1.23
0.82
0.38
0.41
1
0.91
0.58
0.4
1.02
0.9
0.63
JIP(JR)
JA-responsive gene
AT3G16470
0.63
1.67
1.76
0.77
0.37
1.21
1.51
0.93
0.38
1.05
1.25
0.81
Abscisic acid-related genes
RD29A
Desiccation-responsive protein 29A
AT5G52310
0.28
0.6
0.39
0.19
0.24
0.39
0.42
0.28
0.19
0.29
0.33
0.37
RD22
Dehydration-responsive protein 22
AT5G25610
3.04
2.3
1.57
1.16
1.78
1.75
1.12
0.95
2.23
1.55
1.19
1.43
KIN2
Cold-responsive 6.6
AT5G15970
0.78
1.3
1.47
0.53
0.55
1.03
1.25
0.67
0.36
0.74
1.25
0.7
COR47
Cold regulated 47
AT1G20440
0.63
0.94
0.8
0.41
0.48
0.39
0.76
0.48
0.31
0.49
0.74
0.55
RAB18
Responsive ABA 18
AT5G66400
0.49
1.01
0.76
0.33
0.26
0.39
0.59
0.47
0.36
0.45
0.57
0.42
Cell death–related genes
DAD-1
Defender against apoptotic death 1
AT1G32210
0.62
1.28
0.79
0.54
0.3
0.75
0.78
0.59
0.43
0.72
0.68
0.56
LSD1
Lesion simulating disease 1
AT4G20380
0.35
0.85
0.46
0.26
0.19
0.5
0.41
0.34
0.32
0.51
0.42
0.35
ACD1
Accelerated cell death 1
AT3G44880
0.32
0.69
0.53
0.31
0.16
0.46
0.46
0.41
0.22
0.42
0.44
0.35
SAG12
Senescence-associated gene 1
AT5G43060
0.27
0.67
0.54
0.34
0.15
0.29
0.46
0.44
0.26
0.42
0.38
0.35
SRG3
Senescence-related gene 3
AT3G02040
0.26
0.83
0.58
0.27
0.13
0.32
0.46
0.37
0.22
0.46
0.46
0.35
SAP
Sterile apetela
AT4G35770
0.39
1.11
0.87
0.44
0.2
0.41
0.5
0.42
0.24
0.64
0.55
0.5
ATBI-1
Bax inhibitor 1
AT5G47120
0.56
2.49
1.64
0.7
0.28
0.98
1.11
0.66
0.46
1.53
0.84
0.72
CDF1
Cell growth defect factor 1
AT5G23040
0.48
0.94
0.76
0.45
0.29
0.38
0.56
0.35
0.56
0.51
0.47
0.44
Other ozone-response genes
SAMDC
S-adenosylmethionine decarboxylase
AT3G02470
1.64
1.37
0.8
0.54
0.95
0.93
0.76
0.69
0.94
0.82
0.68
0.61
ADC1
Arginine decarboxylase 1
AT2G16500
0.57
0.83
0.38
0.22
0.33
0.49
0.34
0.34
0.32
0.47
0.31
0.25
ADC2
Arginine decarboxylase 2
AT4G34710
0.28
0.87
0.59
0.28
0.18
0.62
0.49
0.35
0.24
0.57
0.58
0.31
SPDS
Spermidine synthase 1
AT1G23820
0.37
0.74
0.6
0.48
0.25
0.37
0.46
0.43
0.35
0.46
0.5
0.46
MPT1
Mitochondrial phosphate transporter 1
AT5G14040
0.45
1
0.69
0.5
0.33
0.4
0.54
0.44
0.47
0.63
0.53
0.46
ANT1
Aromatic neutral amino acid transporter 1
AT3G08580
1.16
1.96
1.31
1.07
0.85
1.11
1.25
0.97
0.71
1.2
0.84
1.17
TPT
Triose-phosphate translocator
AT5G46110
1.88
1.61
0.88
0.71
1.12
0.72
0.65
0.54
1.41
0.95
0.48
0.72
ATOZI1
Ozone- induced protein 1
AT4G00860
0.67
1.34
0.89
0.69
0.46
0.55
0.97
0.59
0.53
0.61
0.49
0.64
SDH2-2
Succinate dehydrogenase 2-2
AT5G40650
0.27
0.4
0.45
0.27
0.26
0.42
0.47
0.39
0.31
0.28
0.38
0.26
SDH1-1
Succinate dehydrogenase 1-1
AT5G66760
0.26
0.51
0.49
0.27
0.23
0.41
0.5
0.44
0.25
0.33
0.37
0.28
ADH1
Alcohol dehydrogenase 1
AT1G77120
0.18
0.44
0.31
0.13
0.13
0.25
0.33
0.24
0.17
0.2
0.23
0.16
G6PD
Glucose-6-phosphate dehydrogenase 2
AT5G13110
0.21
0.55
0.31
0.15
0.11
0.34
0.3
0.25
0.13
0.35
0.3
0.19
14-3-3
General regulatory factor 8
AT5G65430
0.54
0.92
0.54
0.35
0.23
0.6
0.53
0.45
0.33
0.58
0.54
0.45
ELI3-1
Elicitor-activated gene 3
AT4G37980
0.98
0.83
0.76
0.43
0.4
0.64
0.71
0.52
0.49
0.79
0.69
0.56
ICL
Isocitrate lyase
AT3G21720
0.15
0.38
0.28
0.15
0.09
0.31
0.29
0.26
0.15
0.21
0.21
0.15
WAK1
Cell wall-associated kinase 1
AT1G21250
0.14
1
0.86
0.6
0.11
0.51
0.78
0.68
0.14
0.43
0.47
0.44
BCB
Blue copper binding protein
AT5G20230
0.31
4.32
2.59
1.1
0.15
2.05
2.28
1.23
0.29
2.68
2.19
1.12
SPS
Sucrose-phosphate synthase
AT5G20280
0.24
0.44
0.32
0.25
0.13
0.13
0.27
0.29
0.2
0.2
0.17
0.22
SUS1
Sucrose synthase 1
AT5G20830
0.18
0.5
0.36
0.17
0.09
0.18
0.28
0.23
0.17
0.31
0.19
0.14
BFS1
Beta-fructosidase 1
AT3G13790
0.14
1.05
0.75
0.34
0.09
0.54
0.73
0.51
0.18
0.48
0.36
0.25
SUC2
Sucrose-proton symporter 2
AT1G22710
0.39
0.26
0.38
0.17
0.36
0.34
0.3
0.24
0.34
0.24
0.26
0.18
LTI30
Low-temperature-induced protein 30
AT3G50970
0.15
0.37
0.32
0.16
0.16
0.31
0.26
0.22
0.19
0.22
0.24
0.18
LTI29
Low-temperature-induced protein 29
AT1G20450
0.35
0.75
0.52
0.3
0.28
0.42
0.45
0.35
0.27
0.46
0.51
0.39
bZIP60
Basic region/leucine zipper motif 60
AT1G42990
0.38
1.07
0.51
0.27
0.26
0.73
0.51
0.38
0.22
0.75
0.48
0.34
BIP2
Luminal binding protein 2
AT5G42020
0.39
1.1
0.94
0.59
0.23
0.99
0.94
0.69
0.25
0.97
0.87
0.69
BIP1
Luminal binding protein 1
AT5G28540
0.34
0.88
0.9
0.57
0.22
0.8
0.8
0.62
0.3
0.82
0.75
0.64
HSP90
Heat shock protein 90
AT4G24190
0.52
0.94
0.95
0.83
0.33
0.9
0.88
0.75
0.33
0.73
0.76
0.64
HSP81-2
Heat shock protein 81-2
AT5G56030
0.54
2.06
0.65
0.69
0.45
1.04
0.67
0.48
0.48
1.61
0.68
0.62
SRPR
Signal recognition particle receptor
AT2G45770
0.31
0.67
0.38
0.29
0.21
0.3
0.43
0.35
0.27
0.37
0.26
0.23
SEC61α
Protein transport protein
AT2G34250
0.44
1.05
0.75
0.45
0.27
0.62
0.76
0.49
0.34
0.68
0.46
0.4
APX3
Ascorbate peroxidase 3
AT4G35000
0.73
0.9
0.66
0.45
0.54
0.68
0.51
0.59
0.44
0.46
0.59
0.46
APX4
Ascorbate peroxidase 4
AT1G77490
0.65
0.6
0.38
0.24
0.35
0.44
0.46
0.32
0.45
0.35
0.31
0.32
APX5
Ascorbate peroxidase 5
AT4G08390
0.47
0.93
0.57
0.44
0.32
0.49
0.63
0.47
0.49
0.61
0.51
0.47
MDAR1
Monodehydroascorbate reductase 1
AT1G63940
0.21
0.76
0.47
0.26
0.28
0.31
0.47
0.26
0.3
0.36
0.32
0.31
MDAR2
Monodehydroascorbate reductase 2
AT5G03630
0.33
1.79
1
0.33
0.21
1.28
1.11
0.62
0.24
0.82
0.94
0.5
mtDHAR
Mitochondrial dehydroascorbate reductase
AT1G19570
1.16
1.9
2.3
1.16
0.72
1.65
1.61
1.1
0.62
1.47
1.83
1.08
cytDHAR
Cytosolic dehydroascorbate reductase
AT1G75270
0.34
0.92
0.67
0.41
0.25
0.59
0.59
0.6
0.28
0.57
0.44
0.37
DHAR5
Dehydroascorbate reductase 5
AT5G16705
1.02
1.2
0.77
0.6
0.57
0.73
0.6
0.59
0.78
0.73
0.64
0.62
VTC1-1
GDP-mannose pyrophosphorylase
AT2G39770
0.66
1.29
0.65
0.48
0.38
0.89
0.57
0.62
0.34
0.81
0.64
0.51
ATGME
GDP-D-mannose 3',5'-epimerase
AT5G28840
0.78
0.74
0.52
0.44
0.47
0.51
0.43
0.46
0.43
0.43
0.47
0.55
VTC4-1
L-galactose-1-phosphate phosphatase
AT3G02870
0.44
0.82
0.56
0.25
0.26
0.49
0.42
0.37
0.25
0.41
0.45
0.4
L-GalDH
L-galactose dehydrogenase
AT4G33670
0.39
0.67
0.52
0.31
0.23
0.28
0.44
0.35
0.24
0.34
0.38
0.36
ATGLDH
L-galactono-1,4-lactone dehydrogenase
AT3G47930
0.46
0.82
0.57
0.36
0.29
0.32
0.43
0.36
0.46
0.67
0.54
0.48
chlDHAR
Chloroplast dehydroascorbate reductase
AT5G16710
0.6
0.51
0.45
0.34
0.52
0.45
0.27
0.37
0.47
0.37
0.45
0.39
VTC1-c
GDP-mannose pyrophosphorylase
AT4G30570
0.01
0.03
-0.04
0.01
0.01
0.03
0.01
0.07
0.03
0.04
0.05
0.01
AO
L-ascorbate oxidase
AT5G21105
0.12
0.19
0.05
0.08
0.06
0.08
0.04
0.18
0.08
0.2
0.17
0.15
Other ROS scavenging-related genes
PAL1
Phenylalanine ammonia-lyase 1
AT2G37040
0.56
1.91
0.71
0.52
0.36
0.63
0.62
0.59
0.37
0.8
0.56
0.48
4-CL
CoA ligase
AT1G51680
0.54
1.33
0.45
0.35
0.38
0.38
0.49
0.24
0.61
0.53
0.31
0.37
CHS
Chalcone synthase
AT5G13930
0.5
0.46
0.44
0.44
0.43
0.54
0.47
0.64
0.4
0.24
0.47
0.57
GPX1
Glutathione peroxidase 1
AT2G25080
1.37
1.34
0.82
0.53
0.71
0.77
0.77
0.5
1.14
0.78
0.65
0.53
GPX2
Glutathione peroxidase 2
AT2G31570
1.08
1.85
1.23
0.5
0.49
1.33
1.26
0.66
0.56
1.03
0.7
0.76
GPX8
Glutathione peroxidase 8
AT1G63460
0.4
0.91
0.63
0.39
0.22
0.53
0.58
0.56
0.24
0.47
0.48
0.53
GPX3
Glutathione peroxidase 3
AT2G43350
0.55
1.2
0.82
0.53
0.27
0.73
0.68
0.56
0.27
0.6
0.65
0.59
GPX4
Glutathione peroxidase 4
AT2G48150
0.38
0.73
0.42
0.2
0.24
0.28
0.46
0.33
0.18
0.3
0.33
0.27
GPX5
Glutathione peroxidase 5
AT3G63080
0.37
0.97
0.74
0.32
0.24
0.42
0.59
0.37
0.24
0.46
0.43
0.29
GPX6
Glutathione peroxidase 6
AT4G11600
0.44
1.43
1.11
0.44
0.22
0.54
0.6
0.38
0.3
0.85
0.67
0.49
CAT1
Catalase 1
AT1G20630
0.48
0.78
0.57
0.33
0.38
0.43
0.5
0.44
0.42
0.41
0.41
0.54
CAT2
Catalase 2
AT4G35090
3.32
1.3
0.96
0.67
1.72
1.02
0.74
0.89
1.71
0.92
0.7
1.07
CAT3
Catalase 3
AT1G20620
1.75
2.38
1.99
1.19
0.86
1.39
1.32
1.14
0.89
1.25
1.52
1.44
CSD1
Copper, zinc superoxide dismutase 1
AT1G08830
1.51
2.58
1.88
1.36
0.9
1.43
1.45
1.22
0.7
1.32
1.59
1.56
CSD2
Copper, zinc superoxide dismutase 2
AT2G28190
1.75
1.96
1.19
0.85
1.26
0.87
1.04
0.77
0.98
1.18
1.12
1.02
CSD3
Copper, zinc superoxide dismutase 3
AT5G18100
0.51
0.78
0.53
0.3
0.31
0.33
0.42
0.35
0.27
0.4
0.36
0.29
FSD1
Iron superoxide dismutase 1
AT4G25100
0.58
1.11
0.78
0.36
0.2
0.31
0.53
0.38
0.23
0.59
0.39
0.31
FSD2
Iron superoxide dismutase 2
AT5G51100
0.38
0.81
0.43
0.38
0.24
0.28
0.34
0.32
0.25
0.39
0.22
0.26
MSD1
Manganese superoxide dismutase 1
AT3G10920
0.84
1.3
1.07
0.87
0.54
1
0.98
0.85
0.48
0.71
0.87
0.75
PRXC
Class III peroxidase
AT3G49120
0.31
2.48
1.97
1.58
0.2
1.34
2.47
1.87
0.29
1.22
1.16
1.07
ATP2A
Peroxidase 21
AT2G37130
0.4
0.92
0.7
0.59
0.41
0.54
0.64
0.56
0.27
0.42
0.54
0.52
AtMT1
Metallothionein 1
AT3G09390
1.42
1.6
1.56
1.25
0.92
1.05
1.38
1.75
0.75
0.92
1.45
2.17
PⅢ
Protease inhibitor
AT2G02120
0.39
0.8
0.67
0.37
0.29
0.55
0.59
0.43
0.25
0.43
0.65
0.53
TRPA
Tryptophan synthase alpha subunit
AT4G02610
0.23
0.53
0.36
0.21
0.13
0.36
0.31
0.29
0.14
0.26
0.26
0.28
TRPB
Tryptophan synthase beta subunit
AT5G54810
0.39
2.05
0.85
0.44
0.26
0.62
0.81
0.53
0.25
1.09
0.83
0.51
ATP24A
Peroxidase ATP24a
AT5G39580
0.16
0.5
0.28
0.13
0.12
0.21
0.26
0.2
0.12
0.28
0.27
0.16
LECT
Lectin-like protein
AT3G16530
0.25
3.63
1.04
0.24
0.19
1.06
0.69
0.27
0.24
2
0.68
0.38
DHS1
2-dehydro-3-deoxyphosphoheptonate aldolase
AT4G39980
0.22
0.84
0.51
0.24
0.17
0.29
0.4
0.2
0.22
0.49
0.34
0.24
L-GLOLIKE3
L-gulonolactone oxidase like 3
AT2G46750
0.14
0.51
0.32
0.12
0.11
0.2
0.29
0.2
0.12
0.32
0.16
0.17
L-GLOLIKE7
L-gulonolactone oxidase like 7
AT5G56490
0.14
0.49
0.32
0.14
0.11
0.18
0.27
0.15
0.15
0.25
0.16
0.15
Jasmonic acid-relatd genes
VSP1
Vegetative storage protein 1
AT5G24780
0.47
1.63
2.72
1.95
0.3
1.1
1.99
1.04
0.34
1.25
2.57
2.06
PIN2
Plant defensin-fusion protein 2
AT2G02100
0.62
1.36
1.01
0.66
0.31
0.58
0.89
0.42
0.47
0.61
1
0.78
Salicylic acid-related genes
ICS1
Isochorismate synthase 1
AT1G74710
0.16
1.49
0.51
0.13
0.15
0.94
0.82
0.31
0.11
0.38
0.52
0.15
PAL2
Phenylalanine ammonia-lyase 2
AT3G53260
0.44
1.21
0.28
0.15
0.29
0.66
0.38
0.29
0.3
0.57
0.41
0.23
EDS1
Enhanced disease susceptibility 1
AT3G48090
0.18
1.16
0.54
0.29
0.17
0.66
0.72
0.29
0.17
0.33
0.55
0.26
EDS5
Enhanced disease susceptibility 5
AT4G39030
0.08
1.52
0.68
0.11
0.12
0.88
0.91
0.19
0.1
0.41
0.46
0.12
PAD4
Phytoalexin deficient 4
AT3G52430
0.3
1.12
0.62
0.22
0.19
0.94
0.76
0.24
0.2
0.37
0.55
0.27
CPR5
Consititutive expression of PR genes 5
AT5G64930
0.18
0.49
0.19
0.1
0.12
0.29
0.26
0.14
0.13
0.13
0.19
0.14
NPR1
Nonexpresser of PR genes 1
AT1G64280
0.17
0.56
0.29
0.12
0.17
0.41
0.37
0.19
0.13
0.19
0.26
0.15
PR1
Pathogenesis related protein 1
AT2G14610
0.41
1.29
0.7
0.33
0.33
0.48
0.67
0.48
0.28
0.61
0.56
0.38
PR5
Pathogenesis related protein 5
AT1G75040
0.31
1.5
1.03
0.47
0.3
0.52
0.92
0.66
0.28
0.69
0.6
0.47
GSTF6
Glutathione S transferase F6
AT1G02930
0.39
7.48
2.21
0.78
0.4
2.05
2.25
0.79
0.28
2.82
2.16
1.21
PR2
Pathogenesis related protein 2
AT3G57260
0.24
0.77
0.52
0.64
0.16
0.35
0.72
0.48
0.21
0.35
0.44
0.49
PR5K
Similar to receptor serine/threonine kinase
AT1G75800
0.3
1.02
0.55
0.26
0.16
0.37
0.7
0.26
0.27
0.43
0.43
0.37
BAH1
Benzoic acid hypersensitive 1
AT1G02860
0.13
0.53
0.19
0.1
0.13
0.25
0.19
0.24
0.1
0.09
0.18
0.11
NIMIN1
NIM1 interacting 1
AT1G02450
0.18
0.83
0.54
0.2
0.14
0.61
0.54
0.31
0.11
0.21
0.31
0.16
CAD1
Constitutively activated cell death 1
AT1G29690
0.14
0.93
0.31
0.14
0.14
0.5
0.39
0.21
0.15
0.34
0.33
0.16
WRKY70
WRKY DNA-binding protein 70
AT3G56400
0.3
2.42
1.41
0.5
0.22
1.2
1.01
0.48
0.22
0.45
0.71
0.32
Ethylene-related genes
ACS6
ACC Synthase 6
AT4G11280
0.46
0.76
0.31
0.13
0.23
0.6
0.37
0.2
0.29
0.35
0.32
0.19
PR4
Pathogenesis related protein 4
AT3G04720
0.43
1.62
0.77
0.48
0.21
0.72
0.59
0.42
0.28
0.69
0.73
0.82
ETR1
Ethylene receptor 1
AT1G66340
0.16
0.37
0.18
0.07
0.19
0.25
0.26
0.14
0.08
0.09
0.15
0.09
EIN3
Ethylene-insensitive 3
AT3G20770
0.36
0.91
0.62
0.2
0.29
0.34
0.39
0.27
0.25
0.33
0.3
0.22
CTR1
Constitutive triple response 1
AT5G03730
0.43
1.01
0.85
0.36
0.23
0.45
0.68
0.42
0.29
0.52
0.6
0.41
PDF1.2
Plant defensin 1.2
AT5G44420
0.25
1.88
0.89
0.34
0.24
0.55
0.7
0.52
0.2
0.89
0.61
0.5
PR3
Pathogenesis related protein 3
AT3G12500
0.28
1.32
0.58
0.36
0.19
0.38
0.58
0.41
0.25
0.5
0.44
0.49
GSTF2
Glutathione S transferase F2
AT4G02520
0.39
3.48
1.63
0.91
0.27
0.94
1.53
0.57
0.33
1.2
1.36
1.45
SAM1
S-adenosylmethionine synthetase 1
AT1G02500
0.57
1.33
0.8
0.44
0.35
0.92
0.82
0.62
0.36
0.78
0.9
0.51
EIN2
Ethylene-insensitive 2
AT5G03280
0.19
0.1
0.14
0.05
0.11
0.14
0.05
0.09
0.11
0.1
0.15
0.1
EAT1
ACC oxidase 4
AT1G05010
0.65
2.65
1.18
0.61
0.39
1.93
1.45
0.81
0.48
1.1
0.93
0.57
ACO2
ACC oxidase 2
AT1G62380
0.91
1.9
1
0.44
0.45
0.67
0.73
0.52
0.74
0.82
0.93
0.55
ERS1
Ethylene receptor subfamily 1
AT2G40940
0.29
0.59
0.33
0.13
0.15
0.37
0.34
0.2
0.17
0.22
0.32
0.28
ERT2
Ethylene-regulated nuclear protein 2
AT4G20880
0.78
0.71
0.36
0.23
0.25
0.48
0.38
0.31
0.28
0.37
0.35
0.35
RAP2.3
Related to AP2.3
AT3G16770
0.72
1.03
0.66
0.31
0.29
0.59
0.6
0.38
0.46
0.57
0.58
0.44
EREBP-1
Ethylene responsive element binding factor1
AT5G61600
0.37
0.8
0.58
0.18
0.3
0.39
0.47
0.34
0.24
0.36
0.41
0.28
ERF1
Ethylene response factor 1
AT3G23240
0.31
0.88
0.57
0.19
0.19
0.33
0.48
0.32
0.25
0.44
0.37
0.27
ERF3
Ethylene response factor 3
AT2G31230
0.29
0.97
0.61
0.27
0.24
0.34
0.51
0.3
0.23
0.43
0.67
0.48
Glutathione-related genes
GSTF8
Glutathione S transferase F8
AT2G47730
0.97
4.36
1.87
0.66
0.47
1.79
1.49
0.49
0.62
1.6
1.16
0.55
GR1
Gluthatione reductase 1
AT3G24170
0.48
0.79
0.4
0.24
0.35
0.64
0.61
0.46
0.28
0.39
0.42
0.28
GR2
Gluthatione reductase 2
AT3G54660
0.41
0.64
0.34
0.27
0.32
0.4
0.45
0.31
0.38
0.35
0.37
0.28
GSH1
Gamma-glutamylcysteine synthetase
AT4G23100
0.53
1.34
0.97
0.45
0.38
0.61
0.88
0.34
0.48
0.8
0.64
0.44
GSH2
Glutathione synthetase
AT5G27380
0.27
0.95
0.44
0.23
0.2
0.5
0.43
0.51
0.18
0.6
0.44
0.41
ATSERAT2;1
Ser O-acetyltransferase 1
AT1G55920
0.33
0.69
0.43
0.17
0.22
0.26
0.39
0.19
0.26
0.29
0.22
0.19
ATSERAT3;1
Ser O-acetyltransferase 106
AT2G17640
0.28
0.39
0.27
0.16
0.14
0.3
0.3
0.31
0.13
0.25
0.25
0.19
ATSERAT2;2
Ser O-acetyltransferase 3
AT3G13110
0.44
0.85
0.61
0.34
0.35
0.56
0.53
0.47
0.41
0.78
0.62
0.47
ATSERAT3;2
Ser O-acetyltransferase 3;2
AT4G35640
0.24
0.69
0.38
0.19
0.17
0.32
0.37
0.35
0.18
0.33
0.34
0.24
ATSERAT1;1
Ser O-acetyltransferase 52
AT5G56760
0.52
1.03
0.64
0.33
0.31
0.53
0.61
0.5
0.31
0.57
0.59
0.4
Ascorbate-related genes
APX1
Ascorbate peroxidase 1
AT1G07890
1.29
3.05
1.93
1.12
0.89
1.27
1.4
0.63
0.93
1.55
1.1
1.03
APX2
Ascorbate peroxidase 2
AT3G09640
0.15
0.49
0.31
0.17
0.13
0.17
0.38
0.16
0.2
0.21
0.19
0.18