Species & Dataset
Experiment
Foliar ozone injury
  • Arabidopsis thaliana

  • Common name: Thale cress, Mouse-ear cress

  • Family: Brassicaceae

  • Cultivar: Arabidopsis thaliana (ecotype Col-0 and RLD1)

  • Tissue: Rosettes

  • Ozone concentration: 300 ppb at flow rate of 100 ml per minute

  • Ozone exposure: 1 hour

  • Platform: Microarray

  • Year of study: 2012

  • Location: Lancester, UK

Arabidopsis_injury.png

Title:  A stress-specific calcium signature regulating an ozone-responsive gene expression network in Arabidopsis.

 

Summary: Changes in gene expression form a key component of the molecular mechanisms by which plants adapt and respond to environmental stresses. There is compelling evidence for the role of stimulus-specific Ca2+ signatures in plant stress responses. However, our understanding of how they orchestrate the differential expression of stress-induced genes remains fragmentary. We have undertaken a global study of changes in the Arabidopsis transcriptome induced by the pollutant ozone in order to establish a robust transcriptional response against which to test the ability of Ca2+ signatures to encode stimulus-specific transcriptional information. We show that the expression of a set of co-regulated ozone-induced genes is Ca2+-dependent and that abolition of the ozone-induced Ca2+ signature inhibits the induction of these genes by ozone. No induction of this set of ozone-regulated genes was observed in response to H2O2, one of the reactive oxygen species (ROS) generated by ozone, or cold stress, which also generates ROS, both of which stimulate changes in [Ca2+]cyt. These data establish unequivocally that the Ca2+-dependent changes in gene expression observed in response to ozone are not simply a consequence of an ROS-induced increase in [Ca2+]cyt per se. The magnitude and temporal dynamics of the ozone, H2O2, and cold Ca2+ signatures all differ markedly. This finding is consistent with the hypothesis that stimulus-specific transcriptional information can be encoded in the spatiotemporal dynamics of complex Ca2+ signals in plants.

ReferenceShort, E.F., North, K.A., Roberts, M.R., Hetherington, A.M., Shirras, A.D. and McAinsh, M.R., 2012. A stress‐specific calcium signature regulating an ozone‐responsive gene expression network in Arabidopsis. The Plant Journal, 71(6), pp.948-961

AGI Gene Code
Uniprot ID
Bin Code
Bin Name
Functional annotation
Expression
AT2G41100
P25071
30.3
signalling.calcium
TCH3_ATCAL4__TCH3 (TOUCH 3)
AT4G17615
O81445
30.3
signalling.calcium
CBL1_SCABP5__CBL1 (CALCINEURIN B-LIKE PROTEIN 1); calcium ion binding
AT2G43290
O22845
30.3
signalling.calcium
MSS3__MSS3 (MULTICOPY SUPPRESSORS OF SNF4 DEFICIENCY IN YEAST 3); calcium ion binding
AT5G37770
P25070
30.3
signalling.calcium
TCH2_CML24__TCH2 (TOUCH 2); calcium ion binding
AT1G02270
O81916
30.3
signalling.calcium
endonuclease/exonuclease/phosphatase family protein / calcium-binding EF hand family protein
AT5G42380
Q9FIH9
30.3
signalling.calcium
CML37_CML39__CML37/CML39; calcium ion binding
AT5G54490
Q9LSQ6
30.3
signalling.calcium
PBP1__PBP1 (PINOID-BINDING PROTEIN 1); calcium ion binding
AT1G27460
Q9CB03
30.3
signalling.calcium
AT3G57530
Q6NLQ6
30.3
signalling.calcium
CPK32_CDPK32__CPK32 (CALCIUM-DEPENDENT PROTEIN KINASE 32); calmodulin-dependent protein kinase
AT1G18890
Q9M9V8
30.3
signalling.calcium
ATCDPK1_CPK10__ATCDPK1; calmodulin-dependent protein kinase/ kinase/ protein kinase
AT4G09570
Q38869
30.3
signalling.calcium
CPK4__CPK4 (calcium-dependent protein kinase 4); calmodulin-dependent protein kinase/ kinase
AT5G12480
Q38873
30.3
signalling.calcium
CPK7__CPK7 (CALMODULIN-DOMAIN PROTEIN KINASE 7)
AT3G20410
Q38868
30.3
signalling.calcium
CPK9__CPK9 (CALMODULIN-DOMAIN PROTEIN KINASE 9); calmodulin-dependent protein kinase/ kinase
AT1G76040
Q8RWL2
30.3
signalling.calcium
CPK29__CPK29 (calcium-dependent protein kinase 29); calmodulin-dependent protein kinase/ kinase
AT5G66210
Q9FKW4
29.4
protein.postranslational modification.kinase
CPK28__CPK28 (calcium-dependent protein kinase 28)
AT5G24430
Q9FIM9
30.3
signalling.calcium
calcium-dependent protein kinase, putative / CDPK, putative
AT4G36070
Q1PE17
30.3
signalling.calcium
CPK18__CPK18 (calcium-dependent protein kinase 18); calmodulin-dependent protein kinase
AT2G35890
Q9SJ61
30.3
signalling.calcium
CPK25__CPK25 (calcium-dependent protein kinase 25); calmodulin-dependent protein kinase/ kinase
AT4G37640
O81108
30.3
signalling.calcium
ACA2__ACA2 (CALCIUM ATPASE 2); calmodulin binding
AT4G29900
Q9SZR1
30.3
signalling.calcium
ACA10__ACA10 (autoinhibited Ca2+ -ATPase 10); calcium-transporting ATPase/ calmodulin binding
AT3G57330
Q9M2L4
30.3
signalling.calcium
ACA11__ACA11 (AUTOINHIBITED CA2+-ATPASE 11); calcium-transporting ATPase/ calmodulin binding
AT3G63380
Q9LY77
30.3
signalling.calcium
calcium-transporting ATPase, plasma membrane-type, putative / Ca(2+)-ATPase, putative (ACA12)
AT3G22910
Q9LIK7
30.3
signalling.calcium
calcium-transporting ATPase, plasma membrane-type, putative / Ca(2+)-ATPase, putative (ACA13)
AT4G14640
O23320
30.3
signalling.calcium
CAM8__CAM8 (CALMODULIN 8); calcium ion binding
AT3G51920
Q9S744
30.3
signalling.calcium
CAM9__CAM9 (CALMODULIN 9); calcium ion binding
AT2G41110
P0DH97
30.3
signalling.calcium
CAM2_ACAM-2_ATCAL5__CAM2 (CALMODULIN-2); calcium ion binding
AT1G76640
Q9SRE7
30.3
signalling.calcium
calmodulin-related protein, putative
AT3G03000
Q9M8U1
30.3
signalling.calcium
calmodulin, putative
AT2G41410
P30188
30.3
signalling.calcium
calmodulin, putative
AT2G41090
P30187
30.3
signalling.calcium
calmodulin-like calcium-binding protein, 22 kDa (CaBP-22)
AT3G01830
Q9SGI8
30.3
signalling.calcium
calmodulin-related protein, putative
AT3G25600
Q9LI84
30.3
signalling.calcium
calmodulin, putative
AT3G50770
Q8L3R2
30.3
signalling.calcium
calmodulin-related protein, putative
AT1G73805
Q9C9T2
30.3
signalling.calcium
calmodulin binding
AT5G26920
F4K2R6
30.3
signalling.calcium
calmodulin binding
AT1G73805
Q9C9T2
30.3
signalling.calcium
calmodulin binding
AT5G04020
A0A1P8BH59
30.3
signalling.calcium
calmodulin-binding protein-related (PICBP)
AT5G62570
C0SVV6
30.3
signalling.calcium
calmodulin-binding protein
AT3G54570
Q9M1H1
30.3
signalling.calcium
calmodulin-binding protein-related
AT5G04170
Q9FYE4
30.3
signalling.calcium
calcium-binding EF hand family protein
AT3G10300
Q8W4L0
30.3
signalling.calcium
calcium-binding EF hand family protein
AT5G39670
Q93Z27
30.3
signalling.calcium
calcium-binding EF hand family protein
AT4G27280
O81831
30.3
signalling.calcium
calcium-binding EF hand family protein
AT3G47480
Q9SN89
30.3
signalling.calcium
calcium-binding EF hand family protein
AT2G46600
Q9ZPX9
30.3
signalling.calcium
calcium-binding protein, putative
AT1G18210
Q9LE22
30.3
signalling.calcium
calcium-binding protein, putative
AT1G21550
Q9LPK5
30.3
signalling.calcium
calcium-binding protein, putative
AT5G62070
Q9FIT1
30.3
signalling.calcium
IQD23__IQD23 (IQ-domain 23); calmodulin binding
AT3G49260
Q9ASW3
30.3
signalling.calcium
IQD21__IQD21 (IQ-DOMAIN 21, IQ-domain 21); calmodulin binding
AT4G14750
A0A1P8B590
30.3
signalling.calcium
IQD19__IQD19 (IQ-DOMAIN 19); calmodulin binding
AT5G49480
Q9FDX6
30.3
signalling.calcium
ATCP1_ACP1__ATCP1 (CA2+-BINDING PROTEIN 1); calcium ion binding
AT1G52410
F4ICX9
30.3
signalling.calcium
TSA1__TSA1 (TSK-ASSOCIATING PROTEIN 1)
AT1G76650
Q9SRE6
30.3
signalling.calcium
CML38__CML38
AT2G33380
O22788
30.3
signalling.calcium
RD20__RD20 (RESPONSIVE TO DESSICATION 20); calcium ion binding
AT1G08860
Q5XQC7
30.3
signalling.calcium
BON3__BON3 (BONZAI 3)
AT1G08450
O04153
30.3
signalling.calcium
CRT3__CRT3 (CALRETICULIN 3); calcium ion binding
AT4G34150
O49490
30.3
signalling.calcium
C2 domain-containing protein
AT4G33050
O82645
30.3
signalling.calcium
EDA39__EDA39 (embryo sac development arrest 39)