Species & Dataset
  • Arabidopsis thaliana

  • Common name: Thale cress, Mouse-ear cress

  • Family: Brassicaceae

Foliar ozone injury
Arabidopsis_injury.png
Experiment
  • Cultivar: Arabidopsis thaliana (ecotype Col-0 and RLD1)

  • Tissue: Rosettes

  • Ozone concentration: 300 ppb at flow rate of 100 ml per minute

  • Ozone exposure: 1 hour

  • Platform: Microarray

  • Year of study: 2012

  • Location: Lancester, UK

Title:  A stress-specific calcium signature regulating an ozone-responsive gene expression network in Arabidopsis.

 

Summary: Changes in gene expression form a key component of the molecular mechanisms by which plants adapt and respond to environmental stresses. There is compelling evidence for the role of stimulus-specific Ca2+ signatures in plant stress responses. However, our understanding of how they orchestrate the differential expression of stress-induced genes remains fragmentary. We have undertaken a global study of changes in the Arabidopsis transcriptome induced by the pollutant ozone in order to establish a robust transcriptional response against which to test the ability of Ca2+ signatures to encode stimulus-specific transcriptional information. We show that the expression of a set of co-regulated ozone-induced genes is Ca2+-dependent and that abolition of the ozone-induced Ca2+ signature inhibits the induction of these genes by ozone. No induction of this set of ozone-regulated genes was observed in response to H2O2, one of the reactive oxygen species (ROS) generated by ozone, or cold stress, which also generates ROS, both of which stimulate changes in [Ca2+]cyt. These data establish unequivocally that the Ca2+-dependent changes in gene expression observed in response to ozone are not simply a consequence of an ROS-induced increase in [Ca2+]cyt per se. The magnitude and temporal dynamics of the ozone, H2O2, and cold Ca2+ signatures all differ markedly. This finding is consistent with the hypothesis that stimulus-specific transcriptional information can be encoded in the spatiotemporal dynamics of complex Ca2+ signals in plants.

ReferenceShort, E.F., North, K.A., Roberts, M.R., Hetherington, A.M., Shirras, A.D. and McAinsh, M.R., 2012. A stress‐specific calcium signature regulating an ozone‐responsive gene expression network in Arabidopsis. The Plant Journal, 71(6), pp.948-961

Affymetrics probe
Gene
Functional annotation
267083_At
At2g41100
TCH3_ATCAL4__TCH3 (TOUCH 3)
245251_At
At4g17615
CBL1_SCABP5__CBL1 (CALCINEURIN B-LIKE PROTEIN 1); calcium ion binding
266447_At
At2g43290
MSS3__MSS3 (MULTICOPY SUPPRESSORS OF SNF4 DEFICIENCY IN YEAST 3); calcium ion binding
249583_At
At5g37770
TCH2_CML24__TCH2 (TOUCH 2); calcium ion binding
264123_At
At1g02270
endonuclease/exonuclease/phosphatase family protein / calcium-binding EF hand family protein
249197_At
At5g42380
CML37_CML39__CML37/CML39; calcium ion binding
248164_At
At5g54490
PBP1__PBP1 (PINOID-BINDING PROTEIN 1); calcium ion binding
264449_At
At1g27460
NPGR1__NPGR1 (NO POLLEN GERMINATION RELATED 1); calmodulin binding
251636_At
At3g57530
CPK32_CDPK32__CPK32 (CALCIUM-DEPENDENT PROTEIN KINASE 32); calmodulin-dependent protein kinase
261378_At
At1g18890
ATCDPK1_CPK10__ATCDPK1; calmodulin-dependent protein kinase/ kinase/ protein kinase
255039_At
At4g09570
CPK4__CPK4 (calcium-dependent protein kinase 4); calmodulin-dependent protein kinase/ kinase
245156_At
At5g12480
CPK7__CPK7 (CALMODULIN-DOMAIN PROTEIN KINASE 7)
257621_At
At3g20410
CPK9__CPK9 (CALMODULIN-DOMAIN PROTEIN KINASE 9); calmodulin-dependent protein kinase/ kinase
262671_At
At1g76040
CPK29__CPK29 (calcium-dependent protein kinase 29); calmodulin-dependent protein kinase/ kinase
247137_At
At5g66210
CPK28__CPK28 (calcium-dependent protein kinase 28)
249730_At
At5g24430
calcium-dependent protein kinase, putative / CDPK, putative
253128_At
At4g36070
CPK18__CPK18 (calcium-dependent protein kinase 18); calmodulin-dependent protein kinase
263940_At
At2g35890
CPK25__CPK25 (calcium-dependent protein kinase 25); calmodulin-dependent protein kinase/ kinase
253063_At
At4g37640
ACA2__ACA2 (CALCIUM ATPASE 2); calmodulin binding
253702_At
At4g29900
ACA10__ACA10 (autoinhibited Ca2+ -ATPase 10); calcium-transporting ATPase/ calmodulin binding
251649_At
At3g57330
ACA11__ACA11 (AUTOINHIBITED CA2+-ATPASE 11); calcium-transporting ATPase/ calmodulin binding
251176_At
At3g63380
calcium-transporting ATPase, plasma membrane-type, putative / Ca(2+)-ATPase, putative (ACA12)
256833_At
At3g22910
calcium-transporting ATPase, plasma membrane-type, putative / Ca(2+)-ATPase, putative (ACA13)
245257_At
At4g14640
CAM8__CAM8 (CALMODULIN 8); calcium ion binding
252037_At
At3g51920
CAM9__CAM9 (CALMODULIN 9); calcium ion binding
267064_At
At2g41110
CAM2_ACAM-2_ATCAL5__CAM2 (CALMODULIN-2); calcium ion binding
259866_At
At1g76640
calmodulin-related protein, putative
258617_At
At3g03000
calmodulin, putative
266371_At
At2g41410
calmodulin, putative
267076_At
At2g41090
calmodulin-like calcium-binding protein, 22 kDa (CaBP-22)
258947_At
At3g01830
calmodulin-related protein, putative
256755_At
At3g25600
calmodulin, putative
252136_At
At3g50770
calmodulin-related protein, putative
260068_At
At1g73805
calmodulin binding
246821_At
At5g26920
calmodulin binding
260046_At
At1g73805
calmodulin binding
250875_At
At5g04020
calmodulin-binding protein-related (PICBP)
247426_At
At5g62570
calmodulin-binding protein
251872_At
At3g54570
calmodulin-binding protein-related
245694_At
At5g04170
calcium-binding EF hand family protein
259137_At
At3g10300
calcium-binding EF hand family protein
249417_At
At5g39670
calcium-binding EF hand family protein
253915_At
At4g27280
calcium-binding EF hand family protein
252417_At
At3g47480
calcium-binding EF hand family protein
265460_At
At2g46600
calcium-binding protein, putative
256129_At
At1g18210
calcium-binding protein, putative
260881_At
At1g21550
calcium-binding protein, putative
247464_At
At5g62070
IQD23__IQD23 (IQ-domain 23); calmodulin binding
252280_At
At3g49260
IQD21__IQD21 (IQ-DOMAIN 21, IQ-domain 21); calmodulin binding
245574_At
At4g14750
IQD19__IQD19 (IQ-DOMAIN 19); calmodulin binding
248607_At
At5g49480
ATCP1_ACP1__ATCP1 (CA2+-BINDING PROTEIN 1); calcium ion binding
259609_At
At1g52410
TSA1__TSA1 (TSK-ASSOCIATING PROTEIN 1)
259879_At
At1g76650
CML38__CML38
255795_At
At2g33380
RD20__RD20 (RESPONSIVE TO DESSICATION 20); calcium ion binding
264646_At
At1g08860
BON3__BON3 (BONZAI 3)
261692_At
At1g08450
CRT3__CRT3 (CALRETICULIN 3); calcium ion binding
253284_At
At4g34150
C2 domain-containing protein
253414_At
At4g33050
EDA39__EDA39 (embryo sac development arrest 39)