Species & Dataset
  • Arabidopsis thaliana

  • Common name: Thale cress, Mouse-ear cress

  • Family: Brassicaceae

Foliar ozone injury
Arabidopsis_injury.png
Experiment
  • Cultivar : Columbia (ecotype Col-0)

  • Tissue: Rosettes

  • Ozone concentration: 300 ppb

  • Ozone exposure: 6 hours (0800-1400 h)

  • Platform: Microarray analysis

  • Year of study: 2006

  • Location: Perugia, Italia

Title:  Gene expression profiles of O3-treated Arabidopsis plants.

 

Summary: To analyse cellular response to O3, the tolerantArabidopsis thaliana genotype Col-0 was exposed to O3 fumigation (300 ppb) for 6 h and the modulation of gene expression during the treatment (3 h after the beginning of the treatment, T3 h) and the recovery phase (6 h from the end of the treatment, T12 h) assessed by gene chip microarray and real-time reverse transcriptase (RT)-PCR analyses. The Arabidopsis transcriptional profile is complex, as new genes (i.e. reticuline oxidase) and pathways, other than those already reported as O3-responsive, appear to be involved in the O3 response. The steady state transcript levels of several WRKY genes were increased in O3-treated plants and the W-box was the cis -element over-represented in the promoter region of T3 h up-regulated genes. The fact that the W-box element was also over-represented in almost all T3 h induced receptor-like kinases (RLKs) suggests a WRKY-mediated control of RLKs under O3 stress and a mechanicistic similarity with the pathogen-induced transcriptional responses. We investigated the molecular and physiological implications of our findings in relation to O3-induced plant stress response.

Reference: Tosti, N., Pasqualini, S., Borgogni, A., Ederli, L., Falistocco, E., Crispi, S. and Paolocci, F., 2006. Gene expression profiles of O3‐treated Arabidopsis plants. Plant, cell & environment, 29(9), pp.1686-1702.

Gene Locus
Fold change
Functional annotation
At1g30710
n.c
putative reticuline oxidase-like protein similar to GB:P30986 from [Eschscholzia californica] ( berberine bridge-forming enzyme ), ESTs gb|F19886, gb|Z30784 and gb|Z30785 come from this gene
At1g30720
2.8
putative reticuline oxidase-like protein similar to GB:P30986 from [Eschscholzia californica] ( berberine bridge-forming enzyme ), ESTs gb|F19886, gb|Z30784 and gb|Z30785 come from this gene
At1g30740
n.c
putative reticuline oxidase-like protein similar to GB:P30986 from [Eschscholzia californica] ( berberine bridge-forming enzyme ), ESTs gb|F19886, gb|Z30784 and gb|Z30785 come from this gene
At1g30700
n.c.
putative reticuline oxidase-like protein similar to GB:P30986 from [Eschscholzia californica] ( berberine bridge-forming enzyme ), ESTs gb|F19886, gb|Z30784 and gb|Z30785 come from this gene; supported by cDNA: gi_16930506_gb_AF419607.1_AF419607
At1g30760
n.c
putative reticuline oxidase-like protein similar to GB:P30986 from [Eschscholzia californica] ( berberine bridge-forming enzyme ), ESTs gb|F19886, gb|Z30784 and gb|Z30785 come from this gene
At3g28450
1.7
receptor kinase
At2g20850
1.8
putative LRR receptor protein kinase
At1g49730
1.7
hypothetical protein
At1g69270
n.c.
receptor protein kinase, putative
At2g32680
n.c.
putative disease resistance protein
At5g67280
n.c.
receptor-like protein kinase
At4g02420
n.c.
not found
At4g13900
n.c.
putative disease resistance protein
At4g23290
n.c.
serine/threonine kinase - like protein
At3g56370
-2.5
putative protein
At3g45640
2
MPK3, mitogen-activated protein kinase 3 ; supported by cDNA: gi_14423447_gb_AF386961.1_AF386961
At1g01560
3
MPK11, MAP kinase, putative similar to MAP kinase 5 GI:4239889 from [Zea mays]
At4g29810
1.8
MKK2, MAP kinase kinase 2 ; supported by cDNA: gi_14326470_gb_AF385688.1_AF385688
At4g26070
1.7
MKK1, mitogen activated protein kinase kinase (nMAPKK) ;supported by full-length cDNA: Ceres:31259.
At5g03730
1.9
SERINE/THREONINE-PROTEIN KINASE CTR1
At5g67080
6.6
protein kinase-like protein, MAPKKK19
At5g66850
2.4
MAP protein kinase, MAPKKK5 ; supported by cDNA: gi_13430659_gb_AF360242.1_AF360242
At2g31800
2.6
RAF23, putative protein kinase contains a protein kinase domain profile (PDOC00100)
At5g57610
n.c.
RAF35, putative protein contains similarity to protein kinase, RAF23
At3g46930
2.2
RAF43, protein kinase 6-like protein protein kinase 6 - Glycine max,PIR2:S29851
At3g04910
n.c.
ZIK4, putative mitogen activated protein kinase kinase similar to mitogen activated protein kinase kinase GB:AAC32599 [Oryza sativa]
At3g18750
n.c.
ZIK5, mitogen activated protein kinase kinase, putative similar to mitogen activated protein kinase kinase GB:AAC32599 from [Oryza sativa]
At5g41990
2
ZIK6, MAP kinase ; supported by cDNA: gi_15983508_gb_AF424629.1_AF424629
At5g55560
2
ZIK8, putative protein contains similarity to NRK-related kinase
At1g42990
1.8
bZIP transcription factor, putative contains Pfam profile: PF00170: bZIP transcription factor; supported by cDNA: gi_15028322_gb_AY045964.1_
At3g54620
2
Expressed protein ; supported by cDNA: gi_15451129_gb_AY054645.1_
At1g22070
2.7
transcription factor identical to transcription factor GI:304113 from [Arabidopsis thaliana];supported by full-length cDNA: Ceres:33016.
At3g58120
n.c.
putative protein basic leucine zipper transcription activator shoot-forming PKSF1 - Paulownia kawakamii, EMBL:AF046934;supported by full-length cDNA: Ceres:34553.
At1g06850
n.c.
b-Zip DNA binding protein, putative similar to b-Zip DNA binding protein GB:CAB06697 GI:2246376 from [Arabidopsis thaliana]
At2g18160
-2.2
putative bZIP transcription factor contains a bZIP transcription factor basic domain signature (PDOC00036); supported by cDNA: gi_14335073_gb_AY037216.1_
At5g15830
-3.4
bZIP DNA-binding protein-like putative bZIP DNA-binding protein - Capsicum chinense, EMBL:AF127797
At1g18570
7.1
myb factor, putative similar to myb factor GI:1946266 from [Oryza sativa]; supported by cDNA: gi_3941465_gb_AF062887.1_AF062887
At1g06180
2.3
MYB-related protein identical to GB:CAA90748 GI:1263093 from [Arabidopsis thaliana]
At3g23250
5.9
myb-related transcription factor, putative similar to myb-related transcription factor GB:CAA66952 from [Lycopersicon esculentum]
At1g66230
1.7
myb-related transcription factor, putative similar to GI:1430846 from [Lycopersicon esculentum]
At5g15310
-2.5
myb-related protein - like myb-related protein 1, garden petunia, PIR:S26605
At5g67300
-1.7
myb-related protein, 33.3K (pir |S71284) ;supported by full-length cDNA: Ceres:33763.
At3g46130
-2.5
Myb DNA binding protein -like MYB59, Arabidopsis thaliana, EMBL:AF062894; supported by cDNA: gi_9864078_gb_AF272733.1_AF272733
At5g59780
n.c.
MYB27 protein - like MYB27 protein, Arabidopsis thaliana, PIR:T46166; supported by cDNA: gi_3941479_gb_AF062894.1_AF062894
At1g74430
n.c.
putative MYB family transcription factor contains Pfam profile: PF00249 Myb-like DNA-binding domain; similar to N-term of myb GB:CAA72218 [Oryza sativa]; supported by cDNA: gi_15375287_gb_AF217205.2_AF217205
At4g20820
n.c
reticuline oxidase - like protein reticuline oxidase (EC 1.5.3.9) precursor, Eschscholzia californica, PIR2:A41533
At4g20840
n.c
reticuline oxidase - like protein reticuline oxidase precursor, Eschscholzia californica, PIR:A41533
At4g20830
5.9
reticuline oxidase -like protein reticuline oxidase, Eschscholzia californica, PIR:A41533; supported by cDNA: gi_15983492_gb_AF424621.1_AF424621
At1g26420
16
hypothetical protein similar to reticuline oxidase-like protein GB:CAB45850 GI:5262224 from [Arabidopsis thaliana]
At1g26410
6.7
unknown protein similar to reticuline oxidase-like protein GB:CAB45850 GI:5262224 from [Arabidopsis thaliana]
At1g26400
n.c
hypothetical protein similar to reticuline oxidase-like protein GB:CAB45849 GI:5262223 from [Arabidopsis thaliana]
At1g26390
3.8
hypothetical protein similar to reticuline oxidase-like protein GB:CAB45850 GI:5262224 from [Arabidopsis thaliana]; supported by cDNA: gi_15293132_gb_AY051000.1_
At1g26380
23.4
hypothetical protein similar to reticuline oxidase-like protein GB:CAB45850 GI:5262224 from [Arabidopsis thaliana]; supported by cDNA: gi_13430839_gb_AF360332.1_AF360332
At1g01980
n.c
hypothetical protein similar to reticuline oxidase-like protein GB:CAB45849 GI:5262223 from [Arabidopsis thaliana]
At1g11770
n.c
putative reticuline oxidase strong similarity to gb|AF049347 berberine bridge enzyme from Berberis stolonifera
At2g30990
2
hypothetical protein predicted by genefinder
At2g44040
2.5
unknown protein ;supported by full-length cDNA: Ceres:10293.
At2g30250
2
putative WRKY-type DNA binding protein ; supported by cDNA: gi_15027912_gb_AY045813.1_
At2g22970
2.8
putative serine carboxypeptidase II ; supported by cDNA: gi_14517521_gb_AY039596.1_
At2g23770
2.2
putative protein kinase contains a protein kinase domain profile (PDOC00100)
At2g30070
2.5
high affinity K+ transporter (AtKUP1/AtKT1p) identical to GB:AF029876; supported by cDNA: gi_2654087_gb_AF033118.1_AF033118
At2g39920
2.2
hypothetical protein predicted by genscan;supported by full-length cDNA: Ceres:33701.
At2g44370
2.5
unknown protein highly similar to GP|2435515|AF024504
At2g19190
5.3
putative receptor-like protein kinase
At2g33710
2.4
putative AP2 domain transcription factor
At2g02810
2.9
unknown protein ;supported by full-length cDNA: Ceres:35441.
At2g30750
7.8
putative cytochrome P450
At2g30770
9.4
putative cytochrome P450
At2g32920
2.1
putative protein disulfide isomerase ; supported by cDNA: gi_15810003_gb_AY054270.1_
At2g39650
2.3
unknown protein
At2g39660
3.8
putative protein kinase
At4g23190
10.2
serine/threonine kinase - like protein
At3g55950
6.6
receptor kinase - like protein
At1g51890
7.4
light repressible receptor protein kinase, putative
At1g51800
6.3
hypothetical protein
At2g19190
5.3
putative receptor-like protein kinase
At1g09970
5
unknown protein
At5g25930
5
receptor-like protein kinase - like
At4g23280
5.2
serine /threonine kinase - like protein
At1g79680
4.6
wall-associated kinase 2, putative
At4g21400
2.7
serine/threonine protein kinase - like protein
At1g21240
3.9
hypothetical protein
At5g01540
3.2
receptor like protein kinase
At5g46330
2.9
receptor protein kinase - like protein
At4g23210
2.9
serine/threonine kinase - like protein
At3g47090
2.5
receptor kinase-like protein
At4g04220
2.4
putative disease resistance protein
At4g23220
2.8
serine/threonine kinase - like protein
At3g53810
2.6
serine/threonine-specific kinase like protein
At4g29050
2
serine/threonine-specific kinase like protein
At4g33430
2.4
somatic embryogenesis receptor-like kinase -like protein
At1g53430
2.4
receptor-like serine/threonine kinase, putative
At1g73080
1.9
hypothetical protein
At1g53440
2.3
receptor-like serine/threonine kinase
At1g70530
2.4
putative protein kinase
At5g01950
2.2
putative protein
At3g28890
2
disease resistance protein
At3g59700
2.3
serine/threonine-specific kinase lecRK1 precursor
At4g04490
2.3
putative receptor-like protein kinase
At1g11050
2.5
Ser/Thr protein kinase isolog
At4g23250
1.8
protein kinase - like protein
At1g51790
1.7
hypothetical protein
At4g04960
1.9
not found
At4g23230
2.1
putative protein
At5g65240
2.3
receptor-like protein kinase
At2g24180
2.1
putative cytochrome P450 identical to GB:D78604; supported by cDNA: gi_15450907_gb_AY054534.1_
At2g37430
5
putative C2H2-type zinc finger protein likely a nucleic acid binding protein
At2g18690
5.2
unknown protein ; supported by cDNA: gi_14517479_gb_AY039575.1_
At2g40740
4.4
putative WRKY-type DNA binding protein
At2g18660
2
hypothetical protein predicted by genscan
At2g18680
5.2
unknown protein
At2g37940
4.3
unknown protein ; supported by cDNA: gi_16604321_gb_AY058059.1_
At2g28080
2.8
putative glucosyltransferase ; supported by full-length cDNA: Ceres: 13761.
At2g38860
4
unknown protein ;supported by full-length cDNA: Ceres:114031.
At2g02370
2.3
hypothetical protein predicted by genscan and genefinder; supported by cDNA: gi_13605570_gb_AF361611.1_AF361611
At2g02410
2.9
hypothetical protein predicted by genscan
At2g28760
2.5
putative nucleotide-sugar dehydratase
At2g28840
3
putative RING zinc finger ankyrin protein ; supported by cDNA: gi_13926221_gb_AF370581.1_AF370581
At2g02220
4.1
putative protein kinase
At2g02340
2.1
putative phloem-specific lectin
At2g02350
2.9
SKP1 interacting partner 3 (SKIP3), putative almost identical to SKP1 interacting partner 3 GI:10716951 from [Arabidopsis thaliana]
At2g02360
2.2
putative phloem-specific lectin
At2g29460
9.5
putative glutathione S-transferase ; supported by cDNA: gi_14423533_gb_AF387004.1_AF387004
At2g29470
3.4
putative glutathione S-transferase ; supported by cDNA: gi_11096003_gb_AF288185.1_AF288185
At2g29410
2.7
putative zinc transporter
At2g32380
2.3
hypothetical protein predicted by genefinder;supported by full-length cDNA: Ceres:9671.
At2g32250
2.4
Mutator-like transposase similar to MURA transposase of maize Mutator transposon
At2g41380
2.1
putative embryo-abundant protein
At2g43160
2.4
putative clathrin binding protein (epsin)
At2g06925
2.3
Expressed protein ; supported by full-length cDNA: Ceres: 7600.
At2g16900
3.9
hypothetical protein predicted by genefinder; supported by cDNA: gi_14532491_gb_AY039870.1_
At2g23830
2.1
unknown protein
At2g46140
2.9
putative desiccation related protein ; supported by full-length cDNA: Ceres: 2747.
At2g29720
2.1
putative monooxygenase ;supported by full-length cDNA: Ceres:34214.
At2g25735
4
Expressed protein ; supported by full-length cDNA: Ceres: 7152.
At2g25850
2.1
putative poly(A) polymerase ; supported by cDNA: gi_14532699_gb_AY039974.1_
At2g02930
2.6
putative glutathione S-transferase ;supported by full-length cDNA: Ceres:27915.; supported by cDNA: gi_11095995_gb_AF288181.1_AF288181
At2g47000
2.1
putative ABC transporter related to multi drug resistance proteins and P-glycoproteins
At2g47130
2.2
putative alcohol dehydrogenase
At2g29100
2.1
putative ligand-gated ion channel protein
At2g22860
3.2
unknown protein
At2g22880
2.4
hypothetical protein predicted by genefinder
At2g29990
2.8
putative NADH dehydrogenase (ubiquinone oxidoreductase)
At2g26150
4.1
putative heat shock transcription factor
At2g44790
4.7
phytocyanin identical to GB:U90428; supported by full-length cDNA: Ceres: 19760.
At2g18950
2.5
hypothetical protein predicted by genefinder
At2g39480
3
putative ABC transporter related to multi drug resistance proteins and P-glycoproteins
At2g39400
4
putative phospholipase ; supported by cDNA: gi_14532651_gb_AY039950.1_
At2g39210
2.8
nodulin-like protein ; supported by cDNA: gi_16930478_gb_AF419593.1_AF419593
At2g34500
5.7
putative cytochrome P450
At2g38470
4.2
putative WRKY-type DNA binding protein
At2g41160
2.1
unknown protein
At2g41100
2.5
calmodulin-like protein identical to GB:D45848; supported by cDNA: gi_15983405_gb_AF424577.1_AF424577
At2g38290
3.4
putative ammonium transporter
At2g30870
3
glutathione S-transferase identical to GB:D17673; supported by cDNA: gi_443698_dbj_D17673.1_ATHERD13
At1g10070
2.3
tat-binding protein, putative Highly Similar to branched-chain amino acid aminotransferase; Location of EST gb|T44177 and gb|AA395381; supported by cDNA: gi_15293208_gb_AY051038.1_
At2g17720
2.4
putative prolyl 4-hydroxylase, alpha subunit ;supported by full-length cDNA: Ceres:36054.
At2g17740
2.5
unknown protein
At1g08930
2.5
zinc finger protein ATZF1, putative identical to GB:BAA25989; supported by cDNA: gi_3123711_dbj_D89051.1_D89051
At1g08940
3.9
unknown protein Similar to Saccharomyces hypothetical protein YDR051c (gb|Z49209). ESTs gb|T44436,gb|42252 come from this gene
At1g09970
5
unknown protein Similar to A. thaliana receptor-like protein kinase (gb|RLK5_ARATH). ESTs gb|ATTS0475,gb|ATTS4362 come from this gene
At1g62300
5.8
unknown protein similar to putative DNA-binding protein GI:7268215 from [Arabidopsis thaliana]; supported by cDNA: gi_12658409_gb_AF331712.1_AF331712
At1g61370
3.9
receptor protein kinase (IRK1), putative similar to receptor protein kinase (IRK1) GI:836953 from [Ipomoea trifida]
At1g61360
4.5
receptor protein kinase (IRK1), putative similar to receptor protein kinase (IRK1) GI:836953 from [Ipomoea trifida]
At1g61420
2.4
receptor kinase, putative similar to receptor kinase GI:166692 from [Arabidopsis thaliana]
At1g61380
3.9
hypothetical protein similar to putative serine/threonine kinase GI:4585880 from [Arabidopsis thaliana];supported by full-length cDNA: Ceres:13461.
At1g22900
3
putative disease resistance response protein similar to GB:AAD29806
At1g22890
3.4
unknown protein
At1g03740
2.7
putative protein kinase similar to (Z71703), cdc2-like protein kinase; similar to ESTs gb|T20748, gb|T20464, and emb|Z17761; supported by cDNA: gi_14532735_gi_13430451
At1g24140
2.7
putative metalloproteinase similar to GB:AAB61099
At1g24150
2.1
unknown protein
At1g61250
2.7
secretory carrier membrane protein identical to secretory carrier membrane protein GI:7109228 from [Arabidopsis thaliana];supported by full-length cDNA: Ceres:27197.
At1g23090
2.2
putative sulphate transporter protein strongly similar to GB:BAA75015, location of EST gb|W43788 and gb|N96564; supported by cDNA: gi_4579912_dbj_AB023423.1_AB023423
At1g60730
2.7
auxin-induced protein, putative similar to auxin-induced atb2 GI:6562980 from [Arabidopsis thaliana]; supported by cDNA: gi_13605500_gb_AF361576.1_AF361576
At1g76930
2.4
hypothetical protein contains similarity to extensin GI:1165127 from [Nicotiana sylvestris]; supported by cDNA: gi_12248385_dbj_AB031820.1_AB031820
At1g61560
5.6
Mlo protein, putative similar to Mlo protein GI:1877220 from [Hordeum vulgare]; supported by cDNA: gi_14091581_gb_AF369567.1_AF369567
At1g52140
2.2
unknown protein ;supported by full-length cDNA: Ceres:25812.
At1g52040
2.7
myrosinase-binding protein homolog, putative almost identical to myrosinase-binding protein homolog GI:2997767 from [Arabidopsis thaliana]; supported by cDNA: gi_13937207_gb_AF372958.1_AF372958
At1g03850
3.5
hypothetical protein predicted by genefinder;supported by full-length cDNA: Ceres:271253.
At1g55450
2.6
hypothetical protein similar to embryo-abundant protein GB:L47672 GI:1350530 from [Picea glauca]; supported by cDNA: gi_14335021_gb_AY037190.1_
At1g05010
3.6
1-aminocyclopropane-1-carboxylate oxidase Identical to 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase) gb|X66719 (EAT1). ESTs gb|T43073, gb|T5714, gb|R90435, gb|R44023, gb|AA597926, gb|AI099676, gb|AA650810 and gb|29725 come from this gene
At2g02010
9.1
putative glutamate decarboxylase ; supported by cDNA: gi_13605709_gb_AF361836.1_AF361836
At2g43000
4
NAM (no apical meristem)-like protein
At2g06510
2
putative replication protein A1
At2g20900
2.1
putative diacylglycerol kinase ;supported by full-length cDNA: Ceres:15863.
At2g20960
2.1
pEARLI 4 protein Same as GB: L43081; supported by cDNA: gi_871781_gb_L43081.1_ATHPEARA
At2g46620
3.1
hypothetical protein predicted by genefinder
At2g15480
2.6
putative glucosyltransferase
At2g06050
2.5
12-oxophytodienoate-10,11-reductase ; supported by cDNA: gi_15294261_gb_AF410322.1_AF410322
At2g28210
3.8
putative carbonic anhydrase
At2g20145
2.4
Expressed protein ; supported by cDNA: gi_13605516_gb_AF361584.1_AF361584
At2g27310
2.1
unknown protein
At2g13810
2.5
putative aspartate aminotransferase ; supported by cDNA: gi_15982837_gb_AY057526.1_
At2g25470
2
putative disease resistance protein
At2g32210
2.3
unknown protein ;supported by full-length cDNA: Ceres:31665.
At2g32190
2.2
unknown protein ; supported by full-length cDNA: Ceres: 40344.
At2g32240
2.9
putative myosin heavy chain ; supported by cDNA: gi_15982766_gb_AY057490.1_
At2g03540
4
hypothetical protein predicted by genscan
At2g03530
2.4
putative integral membrane protein
At2g32140
2.2
putative disease resistance protein